Bifidobacterium breve DSM 32583 and Limosilactobacillus fermentum CECT5716 postbiotics attenuate S. aureus and IL-33-induced Th2 responses

IF 6.1 1区 生物学 Q1 MICROBIOLOGY Microbiological research Pub Date : 2024-09-21 DOI:10.1016/j.micres.2024.127913
Yacine Amar , Jana Grube , Martin Köberle , Monika Schaubeck , Tilo Biedermann , Thomas Volz
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Abstract

Over the past decades, the prevalence of allergic diseases noticeably increased in industrialized countries. The Th2 immune response plays a central role in these pathologies and its modulation using pro-/postbiotics constitutes a promising approach to prevent or alleviate disease symptoms. The aim of this in vitro study, was to investigate the ability of human milk-derived Bifidobacterium breve DSM 32583 (Bb) and Limosilactobacillus fermentum CECT5716 (Lf), to modulate the Th2 induced responses. To this end, Th2 cells were generated by co-culturing of human naïve Th cells with monocyte-derived dendritic cells (moDCs) either stimulated with Staphylococcus aureus or IL-33. The immunomodulatory effects of pro-/postbiotic preparations of Bb and Lf on moDCs and Th2 cells were evaluated in terms of maturation markers expression and cytokines production. Remarkably, the tested strains induced the anti-inflammatory cytokine IL-10 in moDCs, in a strain-, dose- and viability-dependent manner with no significant upregulation of IL-12p70 nor CD83, CD86 or HLA-DR. Interestingly, Bb and Lf postbiotics were able to dampen the Th2/Th1 response induced upon S. aureus- or IL-33 stimulation. They were also able to synergistically induce IL-10 in moDCs and T cells, upon co-stimulation with LPS. Finally, we observed that live probiotics triggered a mild Th1 response that was attenuated in the presence of galacto-oligosaccharides. Altogether, Bb and Lf pro-/postbiotics exhibited remarkable immune regulatory effects on both moDCs and Th2 cells. Therefore, further in vivo studies should be considered to validate these findings and assess their ability to prevent allergy or alleviate its symptoms in affected patients.
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双歧杆菌(Bifidobacterium breve DSM 32583)和柠檬乳杆菌发酵体(Limosilactobacillus fermentum CECT5716)后益生菌可减轻金黄色葡萄球菌和 IL-33 诱导的 Th2 反应
过去几十年来,工业化国家的过敏性疾病发病率明显上升。Th2 免疫反应在这些病症中起着核心作用,而使用益生菌/后益生菌对其进行调节是预防或减轻疾病症状的一种很有前景的方法。这项体外研究的目的是调查人乳衍生的乳双歧杆菌 DSM 32583(Bb)和低硅乳杆菌发酵体 CECT5716(Lf)调节 Th2 诱导反应的能力。为此,通过用金黄色葡萄球菌或 IL-33 刺激人类幼稚 Th 细胞与单核细胞衍生树突状细胞(moDCs)共培养,产生 Th2 细胞。从成熟标志物表达和细胞因子产生的角度评估了 Bb 和 Lf 的前/后生物制剂对 moDCs 和 Th2 细胞的免疫调节作用。值得注意的是,所测试的菌株能诱导 moDCs 中的抗炎细胞因子 IL-10,其诱导方式与菌株、剂量和存活率有关,而 IL-12p70 或 CD83、CD86 或 HLA-DR 均无明显上调。有趣的是,Bb 和 Lf 后生物能抑制金黄色葡萄球菌或 IL-33 刺激诱导的 Th2/Th1 反应。在 LPS 的共同刺激下,它们还能协同诱导 moDCs 和 T 细胞中的 IL-10。最后,我们观察到活益生菌会引发轻微的 Th1 反应,而这种反应在半乳寡糖的存在下会减弱。总之,Bb 和 Lf 原/后益生菌对 moDCs 和 Th2 细胞都有显著的免疫调节作用。因此,应考虑进一步开展体内研究,以验证这些发现,并评估它们预防过敏或减轻过敏患者症状的能力。
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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