Modulation of M1/M2 Cytokines and Inflammatory Enzymes by Persicaria Species Leaf Extracts in Lipopolysaccharide-stimulated RAW 264.7 Cell Macrophages.

IF 1.1 4区 医学 Q4 IMMUNOLOGY Iranian Journal of Immunology Pub Date : 2024-09-08 DOI:10.22034/iji.2024.102221.2780
Adamu Imam Isa
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Abstract

Background: Investigating the impacts of plant-based substances on the regulation of pro-inflammatory M1 and anti-inflammatory M2 cytokines could have significant implications for immune-related health conditions. Seven Persicaria plant species from sub-Saharan Africa were specifically selected for analysis, based on their traditional use in treating inflammation.

Objective: To investigate the inhibitory effects of methanol leaf extracts from selected plants on enzymes involved in chronic inflammation.

Methods: The inhibition of nitric oxide production, acetylcholinesterase activity, and 15-lipoxygenase activity was assessed using the Griess reagent method, Ellman's colorimetric method, and the ferrous oxidation-xylenol orange assay. The quantity of M1/M2 cytokines released was quantified using a flow cytometer.

Results: At a concentration of 50 µg/mL, the methanol extracts of P. limbata exhibited the highest NO inhibition (97.67%), followed by P. nepalensis (93.06%) and P. setosula (92.78%). The NO inhibition caused by the plant extracts was correlated directly with the decrease in NO release by the LPS-stimulated macrophages. Furthermore, the pro-inflammatory enzyme assays indicated that the methanol extracts of P. setosula exhibited the highest enzyme inhibitory activity (LOX 89.59%, AChE 72.12 %). This was followed by P. limbata (with 92.76% for LOX and 56.93% for AChE) and P. nepalensis (with 88.16% for LOX and 47.17% for AChE). Cytokine assays revealed that the extracts of P. limbata had significant dose-dependent suppressive effects on IFN-γ and TNF-α expression while promoting the secretion of IL-2, IL-4, IL-6, and IL-10.

Conclusion: Extracts of P. limbata contain immunomodulatory compounds that could be further explored as potential remedies to target the molecular drivers of chronic inflammation.

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柿叶提取物对脂多糖刺激的 RAW 264.7 细胞巨噬细胞中 M1/M2 细胞因子和炎症酶的调节作用
背景:研究植物性物质对促炎性 M1 和抗炎性 M2 细胞因子调节作用的影响可能会对免疫相关的健康状况产生重大影响。根据其治疗炎症的传统用途,我们特别选择了撒哈拉以南非洲的 7 种柿树植物进行分析:研究选定植物的甲醇叶提取物对慢性炎症相关酶的抑制作用:方法:采用格里斯试剂法、埃尔曼比色法和亚铁氧化-木酚橙测定法评估对一氧化氮产生、乙酰胆碱酯酶活性和 15-脂氧合酶活性的抑制作用。使用流式细胞仪对释放的 M1/M2 细胞因子数量进行量化:结果:在 50 µg/mL 的浓度下,P. limbata 的甲醇提取物对 NO 的抑制率最高(97.67%),其次是 P. nepalensis(93.06%)和 P. setosula(92.78%)。植物提取物对 NO 的抑制作用与 LPS 刺激巨噬细胞释放 NO 的减少直接相关。此外,促炎酶分析表明,P. setosula 的甲醇提取物具有最高的酶抑制活性(LOX 89.59%,AChE 72.12%)。其次是 P. limbata(LOX 抑制率为 92.76%,AChE 抑制率为 56.93%)和 P. nepalensis(LOX 抑制率为 88.16%,AChE 抑制率为 47.17%)。细胞因子检测显示,肢端金枪鱼提取物对 IFN-γ 和 TNF-α 的表达有显著的剂量依赖性抑制作用,同时促进 IL-2、IL-4、IL-6 和 IL-10 的分泌:结论:蛇床子提取物中含有免疫调节化合物,可作为针对慢性炎症分子驱动因素的潜在疗法进行进一步探索。
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来源期刊
Iranian Journal of Immunology
Iranian Journal of Immunology Medicine-Immunology and Allergy
CiteScore
1.60
自引率
0.00%
发文量
50
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Immunology (I.J.I) is an internationally disseminated peer-reviewed publication and publishes a broad range of experimental and theoretical studies concerned with all aspects of immunology.
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