Development of an Ultrasonic Nebulization System for an Inverse Low Temperature Plasma Ionization Source.

IF 3.1 2区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of the American Society for Mass Spectrometry Pub Date : 2024-12-04 Epub Date: 2024-09-27 DOI:10.1021/jasms.4c00259
Alexandra Pape, Juan F Ayala-Cabrera, Florian Stappert, Florian Uteschil, Cedric Thom, Shinji Yoshioka, Yasushi Terui, Oliver J Schmitz
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Abstract

An effective nebulization and evaporation of a liquid sample, like in liquid chromatography, mass spectrometry (LC-MS) couplings, is an essential requirement for the ionization of analyte molecules in the gas phase by, for example, atmospheric pressure chemical ionization (APCI) or the novel low temperature plasma (LTP)-based ion source. These LTP-based ion sources have recently gained interest in the field of atmospheric pressure ion sources, as they can cover a wide range of polarity and molecular mass. They can be used in combination with separation techniques like liquid chromatography or used as an ambient ion source. However, commercial nebulizer systems are of course not constructed to fit to home-built LTP-based ion sources, and this was one incentive to develop a new nebulization system. Instead of an atmospheric pressure chemical ionization (APCI) nebulizer, two commercial nebulizers were disassembled and remodeled to be used as nebulizing systems in an LC-MS setup using an LTP-based ion source. Based on these results, a novel nebulizer system was subsequently developed. To further improve the degree of ionization, cones to focus the LC eluent spray on the plasma region, heating applications, and auxiliary nitrogen gas for dispersion of the solvent droplets were implemented. The LOD that could be calculated via the rule of three resulted in an average of 2.0 μg/L for the APCI-nebulizer and 41 μg/L for the USN. Both could be reduced to 1.4 and 18 μg/L, respectively, by using a TPI-configuration instead of an iLTP. The linearity was equally good for both types of nebulization devices. The final nebulizer could also be operated with a high water content and flow rates higher than those of the two previous ones, indicating an important improvement step.

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为反向低温等离子体电离源开发超声波雾化系统。
在液相色谱-质谱联用(LC-MS)过程中,对液体样品进行有效的雾化和蒸发是通过大气压化学电离(APCI)或新型低温等离子体(LTP)离子源等方法对气相中的分析分子进行电离的基本要求。这些基于低温等离子体的离子源最近在大气压离子源领域引起了人们的兴趣,因为它们可以涵盖广泛的极性和分子质量。它们可与液相色谱等分离技术结合使用,也可用作环境离子源。不过,商用雾化系统的构造当然无法与自制的基于 LTP 的离子源相匹配,这也是开发新型雾化系统的一个诱因。为了在使用基于 LTP 离子源的 LC-MS 装置中用作雾化系统,我们对两台商用雾化器进行了拆卸和改造,以取代常压化学电离(APCI)雾化器。根据这些结果,随后开发了一种新型雾化器系统。为了进一步提高离子化程度,该系统采用了锥形装置将液相色谱洗脱液喷雾集中在等离子区域、加热装置和用于分散溶剂液滴的辅助氮气。根据三法则计算得出的 LOD 平均值为:APCI-雾化器为 2.0 μg/L,USN 为 41 μg/L。通过使用 TPI 配置而不是 iLTP,两者可分别降至 1.4 和 18 μg/L。两种雾化装置的线性度同样良好。最终的雾化器还可以在高含水量和流量高于前两种雾化器的情况下运行,这表明雾化器有了重大改进。
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来源期刊
CiteScore
5.50
自引率
9.40%
发文量
257
审稿时长
1 months
期刊介绍: The Journal of the American Society for Mass Spectrometry presents research papers covering all aspects of mass spectrometry, incorporating coverage of fields of scientific inquiry in which mass spectrometry can play a role. Comprehensive in scope, the journal publishes papers on both fundamentals and applications of mass spectrometry. Fundamental subjects include instrumentation principles, design, and demonstration, structures and chemical properties of gas-phase ions, studies of thermodynamic properties, ion spectroscopy, chemical kinetics, mechanisms of ionization, theories of ion fragmentation, cluster ions, and potential energy surfaces. In addition to full papers, the journal offers Communications, Application Notes, and Accounts and Perspectives
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