Thyroid Cancer With Autocrine Sialyl-fibronectin Depletion Has a Poor Prognosis due to EMT Progression.

IF 1.6 4区 医学 Q4 ONCOLOGY Anticancer research Pub Date : 2024-10-01 DOI:10.21873/anticanres.17285
Ryo Miyake, Hiroshi Takeyama, Yoshinobu Manome, Muneyuki Koyama, Isao Tabei, Hisashi Shioya, Takashi Kazama, Hiroko Nogi
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Abstract

Background/aim: Elevated blood fibronectin (FN) levels have been observed in various cancers; however, their significance remains controversial. Herein, we measured the levels of sialyl-fibronectin (S-FN), a type of FN secreted by tumor cells, and investigated whether blood S-FN secretion is associated with recurrent metastasis and epithelial-mesenchymal transition (EMT).

Patients and methods: An ELISA system recognizing S-FN was constructed, and the amount of S-FN in blood samples from 63 patients with thyroid carcinoma was measured. The relationship between S-FN secretion and clinical prognosis was also examined. Vimentin immunostaining was performed to identify the mesenchymal status of the cells during EMT.

Results: After 12 years of observation, 17/63 patients had recurrent metastases, including nine cases of lymph node recurrence (LNR) and eight cases of remote metastasis (RM). LNR occurred in 7/39 (17.9%) of S-FN-negative cases, where 4/7 (57.1%) had two or more repeat recurrences. In S-FN-positive cases, LNR was observed in 2/24 cases (8.3%), and no repeat recurrence was observed. For RM, 6/39 (15.4%) patients were S-FN-negative, of which 5/6 (83.3%) had progressive disease even during treatment at metastasis. Of the S-FN-positive cases, RM was observed in 2/24 (8.3%) patients; progressive disease was observed in 1/2 (50.0%) patients. In 9/11 S-FN-negative recurrent metastasis cases (81.8%) and 2/4 S-FN-positive cases (50.0%), many vimentin-positive, FN-secreting cells were found in the interstitial tissue around the tumor.

Conclusion: S-FN-negative thyroid cancer has a poor prognosis because of the progression of EMT associated with increased paracrine FN levels in the stroma.

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甲状腺癌自分泌 Sialyl-纤连蛋白耗竭导致 EMT 进展,预后较差。
背景/目的:在多种癌症中均观察到血液中纤维连接蛋白(FN)水平升高,但其意义仍存在争议。在此,我们测定了由肿瘤细胞分泌的一种纤连蛋白(S-FN)的水平,并研究了血液中 S-FN 的分泌是否与复发性转移和上皮-间质转化(EMT)有关:构建了识别S-FN的ELISA系统,并测定了63例甲状腺癌患者血液样本中S-FN的含量。同时还研究了S-FN分泌与临床预后之间的关系。此外,还进行了Vimentin免疫染色,以确定细胞在EMT过程中的间质状态:经过12年的观察,17/63例患者出现复发转移,其中9例淋巴结复发(LNR),8例远处转移(RM)。在S-FN阴性病例中,7/39(17.9%)患者出现淋巴结复发,其中4/7(57.1%)患者出现两次或两次以上的复发。在 S-FN 阳性病例中,有 2/24 例(8.3%)观察到 LNR,没有观察到重复复发。就 RM 而言,6/39(15.4%)例患者为 S-FN 阴性,其中 5/6(83.3%)例患者即使在转移灶治疗期间疾病仍在进展。在 S-FN 阳性病例中,2/24(8.3%)例患者观察到 RM;1/2(50.0%)例患者观察到疾病进展。在9/11例S-FN阴性复发转移病例(81.8%)和2/4例S-FN阳性病例(50.0%)中,肿瘤周围的间质组织中发现了许多波形蛋白阳性、分泌FN的细胞:结论:S-FN阴性甲状腺癌的预后较差,因为EMT的进展与基质中旁分泌型FN水平的升高有关。
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来源期刊
Anticancer research
Anticancer research 医学-肿瘤学
CiteScore
3.70
自引率
10.00%
发文量
566
审稿时长
2 months
期刊介绍: ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.
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