Proteome of plasma extracellular vesicles as a source of colorectal cancer biomarkers.

Q3 Biochemistry, Genetics and Molecular Biology Biomeditsinskaya khimiya Pub Date : 2024-09-01 DOI:10.18097/PBMC20247005356
N A Soloveva, S E Novikova, T E Farafonova, O V Tikhonova, V G Zgoda, A I Archakov
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Abstract

The search for minimally invasive methods for diagnostics of colorectal cancer (CRC) is the most important task for early diagnostics of the disease and subsequent successful treatment. Human plasma represents the main type of biological material used in the clinical practice; however, the complex dynamic range of substances circulating in it complicates determination of CRC protein markers by the mass spectrometric (MS) method. Studying the proteome of extracellular vesicles (EVs) isolated from human plasma represents an attractive approach for the discovery of tissue-secreted CRC markers. We performed shotgun mass spectrometry analysis of EV samples obtained from plasma of CRC patients and healthy volunteers. This MS analysis resulted in identification of 370 proteins (which were registered by at least two peptides). Stable isotope-free relative quantitation identified 55 proteins with altered abundance in EV samples obtained from plasma samples of CRC patients as compared to healthy controls. Among the EV proteins isolated from blood plasma we found components involved in cell adhesion and the VEGFA-VEGFR2 signaling pathway (TLN1, HSPA8, VCL, MYH9, and others), as well as proteins expressed predominantly by gastrointestinal tissues (polymeric immunoglobulin receptor, PIGR). The data obtained using the shotgun proteomic profiling may be added to the panel for targeted MS analysis of EV-associated protein markers, previously developed using CRC cell models.

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作为结直肠癌生物标志物来源的血浆细胞外囊泡蛋白质组。
寻找微创方法诊断结肠直肠癌(CRC)是早期诊断该疾病并成功治疗的最重要任务。人体血浆是临床实践中使用的主要生物材料类型;然而,血浆中循环物质的动态范围十分复杂,这使得用质谱(MS)方法测定 CRC 蛋白质标记物变得复杂。研究从人体血浆中分离出来的细胞外囊泡 (EV) 的蛋白质组是发现组织分泌的 CRC 标志物的一种有吸引力的方法。我们对从 CRC 患者和健康志愿者血浆中获得的 EV 样品进行了枪式质谱分析。通过质谱分析,我们鉴定出了 370 种蛋白质(至少有两种肽进行了登记)。与健康对照组相比,稳定无同位素相对定量鉴定出 55 种在从 CRC 患者血浆样本中获得的 EV 样品中丰度发生变化的蛋白质。在从血浆中分离出的 EV 蛋白中,我们发现了参与细胞粘附和 VEGFA-VEGFR2 信号通路的成分(TLN1、HSPA8、VCL、MYH9 等),以及主要由胃肠道组织表达的蛋白质(聚合免疫球蛋白受体,PIGR)。利用霰弹枪蛋白质组分析获得的数据可添加到以前利用 CRC 细胞模型开发的靶向 MS 分析 EV 相关蛋白标记物的面板中。
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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
期刊最新文献
Fundamentals of protein chemistry at the Institute of Biomedical Chemistry. In silico and in cellulo approaches for functional annotation of human protein splice variants. Nanowire-based biosensors for solving biomedical problems. Proteome of plasma extracellular vesicles as a source of colorectal cancer biomarkers. Registration of activity of a single molecule of horseradish peroxidase using a detector based on a solid-state nanopore.
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