The structure elucidation, anti-aging and hypoglycemic effects of an O-acetyl mannoglucan from the bulbs of Lanzhou lily

IF 2.5 3区 医学 Q3 CHEMISTRY, MEDICINAL Fitoterapia Pub Date : 2024-09-25 DOI:10.1016/j.fitote.2024.106240
Heping Hui , Hui Jin , Xiaoyan Yang , Xuejun Wang , Bo Qin
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Abstract

An O-acetyl mannoglucan (BHP-1) from Lanzhou lily bulbs was structurally elucidated using partial acid hydrolysis, GC–MS, and 2D NMR techniques (COSY, NOESY, HSQC and HMBC) built on prior research, revealing a backbone of -α-D-(1 → 4)-Glcp-β-D-(1 → 4)-Manp- with the most potential side chains -α-D-(1 → 4)-Glcp-β-D-(1 → 4)-Manp-α-D-(1 → 4)-Glcp-α-D-(1 → Glcp- and -α-D-(1 → 4)-Glcp-β-D-(1 → 4)-Manp-α-D-(1 → Glcp-, attached to O-2 and O-3 of glucose and mannose residues, and featuring O-acetyl groups at O-2 or O-3 position of mannose. The terminal residue was α-D-(1 → Glcp. BHP-1 demonstrated anti-aging and hypoglycemic effects, as assessed by C. elegans model and glycolytic enzyme effect in vitro, respectively. The results showed that BHP-1 dose-dependently prolonged lifespan of C. elegans by 33 % at 4 mg/mL under normal conditions, with greater extensions under thermal and oxidative stress (50 % and 80 % increases, respectively, p < 0.05), which were attributed to enhanced antioxidant enzymes (SOD and CAT) and lowered MDA levels of C. elegans. Additionally, BHP-1 exhibited remarkable inhibition on α-glucosidase (93 %) and moderate inhibition on α-amylase (53 %) at 4 mg/mL, with competitive inhibition of α-glucosidase and mixed non-competitive inhibition of α-amylase, respectively. These potential effects might be linked to BHP-1's diverse sugar linkages, higher content of Glc, and certain O-acetyl contents.
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兰州百合鳞茎中一种 O-乙酰甘露聚糖的结构阐释、抗衰老和降血糖作用。
在先前研究的基础上,利用部分酸水解、气相色谱-质谱和二维核磁共振技术(COSY、NOESY、HSQC 和 HMBC),对兰州百合球茎中的一种 O-乙酰甘露聚糖(BHP-1)进行了结构阐释、揭示了-α-D-(1 → 4)-Glcp-β-D-(1 → 4)-Manp- 的骨架,其最具潜力的侧链是-α-D-(1 → 4)-Glcp-β-D-(1 → 4)-Manp-α-D-(1 → 4)-Glcp-α-D-(1 → Glcp- 和-α-D-(1 → 4)-Glcp-β-D-(1 → 4)-Manp-α-D-(1 → Glcp-、连接到葡萄糖和甘露糖残基的 O-2 和 O-3,并在甘露糖的 O-2 或 O-3 位具有 O-乙酰基。末端残基为 α-D-(1 → Glcp)。BHP-1具有抗衰老和降血糖的作用,分别通过秀丽隐杆线虫模型和体外糖酵解作用进行评估。结果表明,在正常条件下,4 毫克/毫升的 BHP-1 可使 elegans 的寿命延长 33%,而在热应激和氧化应激条件下,BHP-1 可使 elegans 的寿命延长更多(分别延长 50% 和 80%,p<0.05)。
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来源期刊
Fitoterapia
Fitoterapia 医学-药学
CiteScore
5.80
自引率
2.90%
发文量
198
审稿时长
1.5 months
期刊介绍: Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.
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