Investigation of the outbreak of Clostridium perfringens using single nucleotide polymorphism analysis for genotyping in Toyama, Japan, 2023.

IF 1.3 4区 医学 Q4 INFECTIOUS DISEASES Japanese journal of infectious diseases Pub Date : 2024-09-30 DOI:10.7883/yoken.JJID.2024.189
Kazuki Saito, Keiko Kimata, Masanori Watahiki, Junko Isobe, Jun-Ichi Kanatani, Kaho Ikeda, Takashi Takeuchi, Chiharu Matsuzaki, Kazunori Oishi
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Abstract

Clostridium perfringens, which produces C. perfringens enterotoxin (CPE), is a major causative agent of food poisoning owing to its gastrointestinal symptoms. Genotyping is important for identifying the etiological agent in outbreaks of C. perfringens. We attempted to genotype strains isolated from an outbreak of food poisoning in Toyama in 2023 using single nucleotide polymorphism (SNP) analysis. The strains of C. perfringens were isolated from a piece of curry food consumed by all patients and from the feces of the patients and employees. The cpe gene was detected in isolates from patients and curry food. The cpe-negative isolates were found in patients who consumed curry foods and in employees. The results of the SNP analysis suggest that the patient- and curry-derived isolates were likely from the same source but were unlikely to be related to the employee-derived isolates. The results of the SNP and pulsed-field gel electrophoresis (PFGE) analyses were consistent, indicating that the patient- and curry-derived isolates came from the same source. SNP analysis, a whole-genome-based genotyping method, is a promising alternative to the traditional PFGE method. Further studies are needed to accumulate more experience with genotyping using SNP analysis for the epidemiological investigation of outbreaks of C. perfringens.

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利用单核苷酸多态性分析进行基因分型的日本富山梭状芽孢杆菌疫情调查,2023 年。
产生产气荚膜梭菌肠毒素(CPE)的产气荚膜梭菌因其胃肠道症状而成为食物中毒的主要致病菌。基因分型对于确定产气荚膜梭菌爆发的病原体非常重要。我们尝试利用单核苷酸多态性(SNP)分析对 2023 年富山爆发的食物中毒事件中分离出的菌株进行基因分型。从所有患者食用的咖喱食品以及患者和员工的粪便中分离出了产气荚膜杆菌菌株。从患者和咖喱食品中分离出的菌株都检测到了 cpe 基因。而在食用咖喱食品的患者和雇员体内则发现了 cpe 阴性的分离物。SNP 分析结果表明,患者和咖喱中的分离物可能来自同一来源,但与员工中的分离物不太可能有关联。SNP 分析和脉冲场凝胶电泳 (PFGE) 分析的结果一致,表明患者和咖喱分离物来自同一来源。SNP 分析是一种基于全基因组的基因分型方法,是传统 PFGE 方法的一种很有前途的替代方法。在利用 SNP 分析对产气荚膜杆菌疫情进行流行病学调查方面,还需要进一步的研究来积累更多的基因分型经验。
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来源期刊
CiteScore
4.50
自引率
4.50%
发文量
172
审稿时长
2 months
期刊介绍: Japanese Journal of Infectious Diseases (JJID), an official bimonthly publication of National Institute of Infectious Diseases, Japan, publishes papers dealing with basic research on infectious diseases relevant to humans in the fields of bacteriology, virology, mycology, parasitology, medical entomology, vaccinology, and toxinology. Pathology, immunology, biochemistry, and blood safety related to microbial pathogens are among the fields covered. Sections include: original papers, short communications, epidemiological reports, methods, laboratory and epidemiology communications, letters to the editor, and reviews.
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