Quantification of Video Sequences of the Microcirculation: A Comparison Between Automated Analysis Using Analysis Manager and Manual Analysis Using Capillary Mapper

IF 1.9 4区医学 Q3 HEMATOLOGY Microcirculation Pub Date : 2024-09-26 DOI:10.1111/micc.12890

Fabian Müller-Graf, Dominik Wrede, Lena Bork, Gerd Klinkmann, Moritz Flick, Daniel A. Reuter, Amelie R. Zitzmann, Stephan H. Böhm, Susanne Reuter

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Abstract

Objective

Microcirculatory disturbances can contribute to organ dysfunction in patients undergoing major surgeries and critical illness. Incident dark field imaging (CytoCam, Braedius Medical BV, Huizen, Netherlands) provides direct visualization of the microcirculation. To utilize this method in daily clinical practice, automated image analysis is essential. This study aims to compare the automated analysis of recorded microcirculation video sequences using CytoCamTools V2 Analysis Manager (Braedius Medical BV) with established manual analysis using Capillary Mapper (Version 1.4.5, University Hospital Münster, Germany) as reference method.

Methods

Sublingual microcirculation video sequences were recorded in patients undergoing laparotomy at four time points (before surgery, 2 and 6 h after surgery, and on the first postoperative day) using incident dark field imaging. Agreement between automated and manual analysis of total vessel density (TVD), perfused vessel density (PVD), and proportion of perfused vessels (PPV) was compared using intraclass correlation (ICC) and Bland–Altman method.

Results

A total of 336 videos from 30 patients were analyzed. The ICC between the two measurement methods was 0.13 for TVD, 0.14 for PVD, and 0.16 for PPV. Bland–Altman analysis showed mean differences (95% limits of agreement) of 10.46 mm/mm² (−1.73–22.65 mm/mm²) for TVD, 8.25 mm/mm² (−9.88–26.39 mm/mm²) for PVD, and − 3.96% (−59.58%–51.65%) for PPV.

Discussion

Automated microcirculatory analysis using the Analysis Manager did not show clinically acceptable agreement with manual analysis using Capillary Mapper. Consequently, automated video analysis using the Analysis Manager does not appear to be a suitable approach.

Trial Registration: ClinicalTrials.gov identifier: DRKS00020264

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微循环视频序列量化：使用分析管理器进行自动分析与使用毛细管映射器进行手动分析的比较。

目的：微循环障碍可导致接受重大手术和危重病人的器官功能障碍。入射暗场成像（CytoCam，Braedius Medical BV，Huizen，Netherlands）可直接观察微循环。要在日常临床实践中使用这种方法，必须进行自动图像分析。本研究旨在比较使用 CytoCamTools V2 分析管理器（Braedius Medical BV）对录制的微循环视频序列进行的自动分析与使用毛细血管绘图仪（1.4.5 版，德国明斯特大学医院）进行的手动分析（作为参考方法）：在四个时间点（术前、术后 2 小时和 6 小时以及术后第一天）使用入射暗场成像技术记录开腹手术患者的舌下微循环视频序列。使用类内相关（ICC）和布兰-阿尔特曼法比较了自动分析与人工分析在总血管密度（TVD）、灌注血管密度（PVD）和灌注血管比例（PPV）方面的一致性：结果：共分析了 30 名患者的 336 个视频。两种测量方法的 ICC 值分别为：TVD 0.13，PVD 0.14，PPV 0.16。Bland-Altman分析显示，TVD的平均差异（95%的一致限）为10.46 mm/mm2（-1.73-22.65 mm/mm2），PVD为8.25 mm/mm2（-9.88-26.39 mm/mm2），PPV为-3.96%（-59.58%-51.65%）：讨论：使用分析管理器进行的自动微循环分析与使用毛细血管绘图仪进行的手动分析没有显示出临床上可接受的一致性。因此，使用分析管理器进行自动视频分析似乎并不是一种合适的方法：试验注册：ClinicalTrials.gov identifier：DRKS00020264.

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来源期刊

Microcirculation 医学-外周血管病

CiteScore

5.00

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.

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