Transmembrane and coiled-coil domain family 3 gene is a novel target of hepatic peroxisome proliferator-activated receptor γ in fatty liver disease

IF 3.8 3区 医学 Q2 CELL BIOLOGY Molecular and Cellular Endocrinology Pub Date : 2024-09-24 DOI:10.1016/j.mce.2024.112379
Daisuke Aibara, Ai Sakaguchi, Kimihiko Matsusue
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Abstract

The peroxisome proliferator-activated receptor gamma (PPARγ) is a nuclear receptor abundantly expressed in the nonalcoholic fatty liver disease (NAFLD). In this study, we investigated the mechanism by which PPARγ regulates the transmembrane and coiled-coil domain family 3 (Tmcc3) gene in the liver. We found that TMCC3 is highly expressed in the fatty liver of humans and mice with NAFLD and alcoholic fatty liver disease. Three exon 1 variants (Tmcc3-1a, -1b, and -1c) of mouse Tmcc3 were identified. TMCC3-1B was highly expressed in the fatty liver of type 2 diabetic ob/ob mice; however, this increase in expression was ameliorated by liver-specific knockout of PPARγ. Reporter assays and electrophoretic mobility shift assays showed that PPARγ positively regulates Tmcc3-1b and -1c transcription through the same PPARγ-responsive element present in the 5′-region of each Tmcc3. Altogether, our results indicate that Tmcc3 is a novel PPARγ target in the fatty liver disease.

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跨膜和盘绕线圈结构域家族 3 基因是脂肪肝中肝脏过氧化物酶体增殖激活受体 γ 的新靶点。
过氧化物酶体增殖激活受体γ(PPARγ)是一种在非酒精性脂肪肝(NAFLD)中大量表达的核受体。本研究探讨了 PPARγ 对肝脏中跨膜和盘绕线圈结构域家族 3(Tmcc3)基因的调控机制。我们发现,TMCC3 在患有非酒精性脂肪肝和酒精性脂肪肝的人类和小鼠的脂肪肝中高表达。我们发现了小鼠 Tmcc3 的三个外显子 1 变体(Tmcc3-1a、-1b 和-1c)。TMCC3-1B在2型糖尿病肥胖/ob小鼠的脂肪肝中高表达;然而,这种表达的增加在肝特异性敲除PPARγ后得到改善。报告实验和电泳迁移实验表明,PPARγ通过存在于每个Tmcc3的5'区的相同的PPARγ反应元件正向调节Tmcc3-1b和-1c的转录。总之,我们的研究结果表明,Tmcc3是脂肪肝中一个新的PPARγ靶点。
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来源期刊
Molecular and Cellular Endocrinology
Molecular and Cellular Endocrinology 医学-内分泌学与代谢
CiteScore
9.00
自引率
2.40%
发文量
174
审稿时长
42 days
期刊介绍: Molecular and Cellular Endocrinology was established in 1974 to meet the demand for integrated publication on all aspects related to the genetic and biochemical effects, synthesis and secretions of extracellular signals (hormones, neurotransmitters, etc.) and to the understanding of cellular regulatory mechanisms involved in hormonal control.
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