Transient Expression of Nicotiana tabacum Silicon-Induced Histidine-Rich Defensins in Nicotiana benthamiana Limits Necrotic Lesion Development Caused by Phytopathogenic Fungi.

IF 2.6 2区农林科学 Q2 PLANT SCIENCES Phytopathology Pub Date : 2024-09-30 DOI:10.1094/PHYTO-05-24-0162-R

Stephen Muhindi, Wendy Zellner, Shin-Yi Marzano, Jennifer Boldt, Scott Leisner

{"title":"Transient Expression of Nicotiana tabacum Silicon-Induced Histidine-Rich Defensins in Nicotiana benthamiana Limits Necrotic Lesion Development Caused by Phytopathogenic Fungi.","authors":"Stephen Muhindi, Wendy Zellner, Shin-Yi Marzano, Jennifer Boldt, Scott Leisner","doi":"10.1094/PHYTO-05-24-0162-R","DOIUrl":null,"url":null,"abstract":"Silicon (Si) supplementation permits plants to better deter infection. Supplementing hydroponically-propagated Nicotiana tabacum with 1 mM potassium silicate (K2SiO3) reduced necrotic lesion development on detached leaves by both Botrytis cinerea and Sclerotinia sclerotiorum. Previously, a family of Si-induced genes was identified in N. tabacum. These genes were members of the Solanaceous Histidine-Rich Defensin (HRD) superfamily and were termed NtHRD1s (the first identified family of Nicotiana tabacum Histidine-Rich Defensins). Defensins were originally identified to participate in innate immunity. Thus, the NtHRD1s were tested for antimicrobial effects on plant pathogens. Transient expression of NtHRD1 genes within Nicotiana benthamiana leaves restricted the development of necrotic lesions caused by B. cinerea and S. sclerotiorum. Thus, the NtHRD1s may be an additional Si-responsive factor conferring beneficial effects on plants.","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-05-24-0162-R","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Silicon (Si) supplementation permits plants to better deter infection. Supplementing hydroponically-propagated Nicotiana tabacum with 1 mM potassium silicate (K₂SiO₃) reduced necrotic lesion development on detached leaves by both Botrytis cinerea and Sclerotinia sclerotiorum. Previously, a family of Si-induced genes was identified in N. tabacum. These genes were members of the Solanaceous Histidine-Rich Defensin (HRD) superfamily and were termed NtHRD1s (the first identified family of Nicotiana tabacum Histidine-Rich Defensins). Defensins were originally identified to participate in innate immunity. Thus, the NtHRD1s were tested for antimicrobial effects on plant pathogens. Transient expression of NtHRD1 genes within Nicotiana benthamiana leaves restricted the development of necrotic lesions caused by B. cinerea and S. sclerotiorum. Thus, the NtHRD1s may be an additional Si-responsive factor conferring beneficial effects on plants.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

在烟草根中瞬时表达烟草硅诱导的富组氨酸防御素，可限制由植物病原真菌引起的坏死性病变的发展。

补充硅（Si）可使植物更好地阻止感染。在水培烟草中添加 1 mM 硅酸钾（K2SiO3）可减少灰霉病菌和硬皮病菌在脱落叶片上造成的坏死病变。此前，在 N. tabacum 中发现了一个 Si- 诱导基因家族。这些基因是茄科组氨酸富防御素（HRD）超家族的成员，被称为 NtHRD1s（第一个确定的烟草组氨酸富防御素家族）。防御素最初被认定参与先天性免疫。因此，我们测试了 NtHRD1s 对植物病原体的抗菌作用。NtHRD1 基因在烟草叶片中的瞬时表达限制了由 B. cinerea 和 S. sclerotiorum 引起的坏死病变的发展。因此，NtHRD1s 可能是对植物有益的另一种 Si- 反应因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Phytopathology 生物-植物科学

CiteScore

5.90

自引率

9.40%

发文量

505

审稿时长

4-8 weeks

期刊介绍： Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.