The influence of fetal bovine serum concentration on stemness and neuronal differentiation markers in stem cells from human exfoliated deciduous teeth

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-09-24 DOI:10.1016/j.tice.2024.102571

Mateus de Oliveira Lisboa , Ana Helena Selenko , Agner Henrique Dorigo Hochuli , Alexandra Cristina Senegaglia , Letícia Fracaro , Paulo Roberto Slud Brofman

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Abstract

Dental Stem Cells (DSCs) from discarded teeth are a non-invasive and ethically favorable source with the potential for neurogenesis due to their ectodermal origin. Stem cells from human exfoliated deciduous teeth (SHED) are particularly promising due to their high differentiation potential and relative immaturity compared to other Mesenchymal Stromal Cells (MSCs). Markers like CD56 and CD271 are critical in identifying MSC subpopulations for therapeutic applications because of their roles in neurodevelopment and maintaining stemness. This study investigates how fetal bovine serum (FBS) concentrations affect the expression of CD56 and CD271 in SHED, influencing their stemness and neuronal differentiation potential. SHEDs were isolated from various donors, cultured, and characterized for MSC traits using markers such as CD14, CD19, CD29, CD34, CD45, CD73, CD90, CD105, CD56, and CD271. Culturing SHED in different FBS conditions (standard 15 %, reduced 1 % and 5 %, and FBS-free) showed that lower FBS concentrations increase CD271 and CD56 expression while maintaining the standard MSC immunophenotype. Importantly, the enhanced expression of these markers can be induced even after SHEDs have been expanded in standard FBS concentrations. These findings suggest that FBS concentration can optimize SHED culture conditions, enhancing their suitability for regenerative medicine and tissue engineering applications.

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胎牛血清浓度对人类脱落牙齿干细胞的干性和神经元分化标记的影响

从废弃牙齿中提取的牙齿干细胞（DSCs）是一种非侵入性且符合道德规范的来源，由于其来源于外胚层，因此具有神经发生的潜力。与其他间充质基质细胞（MSCs）相比，来自人类脱落牙齿（SHED）的干细胞具有高分化潜能和相对不成熟性，因此特别有前景。由于CD56和CD271等标记物在神经发育和保持干性方面的作用，它们对鉴定治疗用间叶干细胞亚群至关重要。本研究探讨了胎牛血清（FBS）浓度如何影响SHED中CD56和CD271的表达，从而影响它们的干性和神经元分化潜能。研究人员从不同的供体中分离出SHED，对其进行培养，并使用CD14、CD19、CD29、CD34、CD45、CD73、CD90、CD105、CD56和CD271等标记物对其间叶干细胞特征进行鉴定。在不同的 FBS 条件（标准 15%、降低 1%、5% 和无 FBS）下培养 SHED 表明，较低的 FBS 浓度会增加 CD271 和 CD56 的表达，同时保持标准的间充质干细胞免疫表型。重要的是，即使在标准 FBS 浓度下扩增 SHED 后，也能诱导这些标记物的表达增强。这些发现表明，FBS浓度可以优化SHED的培养条件，提高它们在再生医学和组织工程应用中的适用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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