Effects and mechanisms of Salvia miltiorrhiza Bunge extract on myocardial cell apoptosis in rat heart failure model.

Acta cirurgica brasileira Pub Date : 2024-09-30 eCollection Date: 2024-01-01 DOI:10.1590/acb396524

Xiaofang Yang, Xuebin Zheng, Xiangqian Xiao, Li Li

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Abstract

Purpose: This work aimed to investigate the effects of Tanshinone IIA (Tan IIA) on myocardial cell (MC) apoptosis in a rat model of heart failure (HF).

Methods: Tan IIA was extracted from Salvia miltiorrhiza Bunge (SMB) using an ethanol reflux method. Fifty rats were randomly divided into five groups: sham (no treatment), mod (HF model establishment), low dose (LD: 0.1 mL/kg Tan IIA), medium dose (MD: 0.3 mL/kg Tan IIA), and high dose (HD: 0.5 mL/kg Tan IIA), with 10 rats in each group. The effects of different doses of Tan IIA on cardiac function, MC apoptosis, and the levels of proteins associated with the PI3K/Akt/mTOR signaling pathway were compared.

Results: Mod group showed a significant decrease in systolic arterial pressure, mean arterial pressure, heart rate, left ventricular systolic pressure, left ventricular ejection fraction, left ventricular fractional shortening, and the levels of p-PI3K, p-Akt, and p-mTOR proteins versus sham group (p < 0.05). Additionally, the left ventricular end-diastolic diameter (LVIDd), end-systolic diameter, diastolic pressure, and MC apoptosis were significantly increased (p < 0.05). LD, MD, and HD groups exhibited significant improvements across various indicators of cardiac function and MC apoptosis versus mod group (p < 0.05).

Conclusions: Tan IIA may improve cardiac function and inhibit MC apoptosis in rats with HF by modulating the PI3K/Akt/mTOR signaling pathway.

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丹参提取物对大鼠心衰模型心肌细胞凋亡的影响及机制

目的：本研究旨在探讨丹参酮 IIA（Tan IIA）对心力衰竭（HF）大鼠模型中心肌细胞（MC）凋亡的影响：方法：丹参酮 IIA 采用乙醇回流法从丹参（SMB）中提取。将 50 只大鼠随机分为五组：假组（不治疗）、模型组（建立 HF 模型）、低剂量组（LD：0.1 mL/kg Tan IIA）、中剂量组（MD：0.3 mL/kg Tan IIA）和高剂量组（HD：0.5 mL/kg Tan IIA），每组 10 只。比较了不同剂量 Tan IIA 对心脏功能、MC 细胞凋亡和 PI3K/Akt/mTOR 信号通路相关蛋白水平的影响：结果：与假组相比，Mod 组的收缩压、平均动脉压、心率、左室收缩压、左室射血分数、左室缩径以及 p-PI3K、p-Akt 和 p-mTOR 蛋白水平均明显下降（p < 0.05）。此外，左心室舒张末期直径（LVIDd）、收缩末期直径、舒张压和 MC 细胞凋亡均显著增加（P < 0.05）。LD组、MD组和HD组的心功能和心肌细胞凋亡的各项指标与mod组相比均有明显改善（P < 0.05）：Tan IIA可通过调节PI3K/Akt/mTOR信号通路，改善高血脂大鼠的心功能并抑制MC凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta cirurgica brasileira

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