Anand Seethepalli, Chanae Ottley, Joanne Childs, Kevin R. Cope, Aubrey K. Fine, John H. Lagergren, Udaya Kalluri, Colleen M. Iversen, Larry M. York
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在农业和自然系统中，根对确定植物生产力和土壤碳输入量非常重要。有时，一个样本中的根的数量太多，无法容纳在一张扫描图像中，因此样本要分几次扫描，而目前还没有汇总数据的标准方法。在此，我们介绍并验证了两种在多个扫描中进行标准化测量的方法：图像连接和统计汇总。我们开发了一个 Python 脚本，可以识别哪些图像属于同一个样本，并返回一个更大的合并图像。我们使用免费开源软件 RhizoVision Explorer 处理这些合并图像和原始图像。开发的 R 脚本可识别属于同一样本的数据行，并应用正确的统计方法返回每个样本的单一数据行。我们使用来自北方泥炭地和北极地区的开关草、杨树以及各种乔木和栎类灌木物种的示例图像对这两种方法进行了比较。除了中位直径无法通过统计汇总准确计算外，这两种方法的大多数根部测量结果几乎相同。我们相信这些方法的可用性将对根生物学界有所帮助。
期刊介绍：
New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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