A clickable coenzyme A derived probe for investigating phosphopantetheinyl transferase activity in natural product biosynthesis†

IF 2.7 3区化学 Q1 CHEMISTRY, ORGANIC Organic & Biomolecular Chemistry Pub Date : 2024-10-10 Epub Date: 2024-10-07 DOI:10.1039/d4ob01485e

Zhiyong Yin , Houchao Xu , Jeroen S. Dickschat

引用次数: 0

Abstract

Phosphopantetheinyl transferases activate carrier proteins through attachment of a coenzyme A derived phosphopantetheinyl linker. This study describes a method to monitor this process through a modified HSCoA with an alkyne group, allowing for the Cu-catalysed alkyne–azide cycloaddition of a fluorescent tag. Application of the method in an enzyme screening resulted in the identification of new promiscuous PPTases.

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一种可点击的辅酶 A 衍生物探针，用于研究天然产物生物合成过程中磷酸泛乙烯基转移酶的活性。

磷酸泛硫乙烯基转移酶通过连接辅酶 A 衍生的磷酸泛硫乙烯基连接体来激活载体蛋白。本研究介绍了一种通过带有炔基的改性 HSCoA 监控这一过程的方法，该方法允许铜催化的炔吖啶环加成荧光标签。在酶筛选中应用这种方法，发现了新的杂合 PPT 酶。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Organic & Biomolecular Chemistry 化学-有机化学

CiteScore

5.50

自引率

9.40%

发文量

1056

审稿时长

1.3 months

期刊介绍： Organic & Biomolecular Chemistry is an international journal using integrated research in chemistry-organic chemistry. Founded in 2003 by the Royal Society of Chemistry, the journal is published in Semimonthly issues and has been indexed by SCIE, a leading international database. The journal focuses on the key research and cutting-edge progress in the field of chemistry-organic chemistry, publishes and reports the research results in this field in a timely manner, and is committed to becoming a window and platform for rapid academic exchanges among peers in this field. The journal's impact factor in 2023 is 2.9, and its CiteScore is 5.5.

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