A Study of the Effect of Sodium-μ2-Dithiosulphate-Tetranitrosyl Diferrate Tetrahydrate on the Structure of Mitochondrial Membranes Isolated from Epicotyls of Pea Seedlings

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology Biophysics Pub Date : 2024-09-27 DOI:10.1134/S0006350924700271

N. Yu. Gerasimov, O. V. Nevrova, I. V. Zhigacheva, N. I. Krikunova, A. K. Vorobyova, I. P. Generozova, A. N. Goloshchapov

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Abstract

Nitric oxide is a signaling molecule of plants under stressful conditions; this molecule can also have a toxic effect. A study of the dose-dependent effect of the nitric oxide donor sodium-μ2-dithiosulphate-tetranitrosyl diferrate tetrahydrate on the structural state of mitochondrial membranes of epicotyles of pea seedlings has been carried out. Upon treating mitochondria with this drug at a concentration of 10^–8 M, thermally induced structural transitions were observed at temperatures from 15°C to 21°C and from 30°C to 36°C in lipid regions, and at temperatures from 12°C to 15°C and from 27°C to 33°C in the protein regions of the membranes. The drug showed antioxidant properties in the lipid regions of the membranes, leading to the accumulation of long unsaturated fatty acids. Treatment of mitochondria with sodium-μ2-dithiosulphate-tetranitrosyl diferrate tetrahydrate at a dose of 10^–4 M caused a significant increase in the level of lipid peroxidation and the fluidity of the lipid phase of membranes.

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从豌豆幼苗外胚叶中分离的线粒体膜结构对四水合钠-μ2-二硫代硫酸盐-四亚硝基二重酸盐影响的研究

一氧化氮是植物在胁迫条件下的一种信号分子；这种分子也有毒性作用。我们研究了一氧化氮供体钠-μ2-二硫代硫酸钠-四亚硝基二甲酸酯四水合物对豌豆幼苗外胚层线粒体膜结构状态的剂量依赖性影响。用浓度为 10-8 M 的这种药物处理线粒体时，在 15°C 至 21°C 和 30°C 至 36°C 的温度下，在脂质区域观察到热诱导的结构转变；在 12°C 至 15°C 和 27°C 至 33°C 的温度下，在膜的蛋白质区域观察到热诱导的结构转变。该药物在膜的脂质区域显示出抗氧化特性，导致长不饱和脂肪酸的积累。用剂量为 10-4 M 的钠-μ2-二硫代硫酸钠-二水合四亚硝基二酸酯处理线粒体，可显著提高脂质过氧化水平和膜脂相的流动性。

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

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1.20

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0.00%

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期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.