In-depth genome-wide characterization of MaNAC25 and MaNAC28 cold-responsive transcription factor binding sites in banana via DAP-Seq

IF 5.4 Q1 PLANT SCIENCES Current Plant Biology Pub Date : 2024-10-06 DOI:10.1016/j.cpb.2024.100389
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Abstract

Mapping transcription factor proteins' binding sites across the entire genome in banana is crucial for unveiling their transcriptional regulatory mechanisms and enhancing our understanding of their regulatory networks. Our study showed that DAP-Seq experiments identified MaNAC25 and MaNAC28 numerous binding peaks, mainly in the promoter regions, with strong signals near the transcription start site (TSS). Significantly, the discovery of new binding motifs for MaNAC28 excluding NAC core binding element CGTA/G indicates their potential as novel DNA binding motifs for NAC transcription factors in cold stress response. Moreover, MaNAC25 was found to chiefly influence biological processes and molecular functions, whereas MaNAC28 was more focused on molecular functions. Both MaNAC25 and MaNAC28 extended their regulatory networks by interacting with other transcription factors during cold stress. Therefore, DAP-Seq technology furnishes essential insights and a robust foundation for researching transcriptional regulatory mechanisms among diverse transcription factors and broadening their regulatory networks.
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通过 DAP-Seq 深入分析香蕉中 MaNAC25 和 MaNAC28 冷响应转录因子结合位点的全基因组特征
绘制香蕉全基因组转录因子蛋白结合位点图对于揭示其转录调控机制和加深对其调控网络的理解至关重要。我们的研究表明,DAP-Seq实验发现了MaNAC25和MaNAC28的许多结合峰,这些结合峰主要位于启动子区域,在转录起始位点(TSS)附近有强信号。值得注意的是,MaNAC28的新结合基团不包括NAC核心结合元件CGTA/G,这表明它们有可能成为NAC转录因子在冷胁迫响应中的新DNA结合基团。此外,研究还发现MaNAC25主要影响生物过程和分子功能,而MaNAC28则更侧重于分子功能。在冷胁迫过程中,MaNAC25和MaNAC28都通过与其他转录因子相互作用来扩展其调控网络。因此,DAP-Seq技术为研究不同转录因子之间的转录调控机制和拓宽其调控网络提供了重要的启示和坚实的基础。
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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