An in vitro method for assay of hormone or agonist-stimulated lipase activity

Abstract

An assay for $\text{in vitro}$ measurement of hormone or agonist-stimulated lipase activity, using exogenous prostaglandin synthetase as a trap for liberated arachidonic acid is described. Fifteen of twenty-six hormones and agonists previously tested $\text{in vivo}$, were tested $\text{in vitro}$ and either stimulated or inhibited release of arachidonic acid parallel to their activity $\text{in vivo}$. Vasopressin stimulated the release of arachidonic acid in ⁻⁵ linear, log-dose manner, from 10⁻¹¹ M to 10⁻⁵ M.