Highly sequence-specific, timing-controllable m6A demethylation by modulating RNA-binding affinity of m6A erasers†

Abstract

Recent advancements in tools using programmable RNA binding proteins and m⁶A-erasers enable sequence-selective and timing-controllable m⁶A demethylation. However, off-target effects are still a concern. This study addresses the problem by reducing the RNA-binding ability of m⁶A-erasers. The modulated m⁶A-erasers achieved sequence-specific and timing-controllable m⁶A demethylation with minimal off-target activity.