Metabolite from supernatant of soil and plant-associated bacteria control biofilm of fish pathogens.

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES BMC Research Notes Pub Date : 2024-10-17 DOI:10.1186/s13104-024-06974-7

Vanessa Everly, Diana Elizabeth Waturangi, Nurmaya Papuangan, Nurhasanah, Pande Gde Sasmita Julyantoro

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Abstract

Objectives: This research aimed to identify and quantify the antibiofilm activity of bioactive compounds from bacteria isolated from rhizosphere and nodule butterfly pea (Clitoria ternatea), rhizosphere clove afo 3 (Syzygium aromaticum), nodule mimosa (Mimosa pudica L.), and soil from gold mining land which were recovered from Ternate, Tidore, Obi Island, and Marotai Island, Eastern part of Indonesia.

Results: Eight supernatants from soil and plant-associated bacteria were found to have quorum quenching activity against Chromobacterium violaceum. All supernatants exhibited antibiofilm activity against biofilm formed by Aeromonas hydrophila and Vibrio harveyi. The supernatant of FT5 showed the highest activity in disrupting (66.59%) and inhibiting (85.63%) the biofilm of A. hydrophila. For V. harveyi, the supernatant of PTM3 showed the highest disruption activity (72.61%), whileRCA7 showed the highest inhibition activity(75.68%). The Gas Chromatography-Mass Spectrometry (GC-MS) identified fatty acids, ester, and diketopiperazine as the compounds related to the antibiofilm activity. Molecular identification revealed that the isolates belong to the genera Bacillus, Priestia, and Chryseobacterium.

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土壤和植物相关细菌上清液中的代谢物可控制鱼类病原体的生物膜。

研究目的本研究旨在鉴定和量化从印度尼西亚东部特尔纳特、蒂多雷、奥比岛和马罗泰岛的金矿开采地的根瘤和蝶形豌豆、根瘤丁香、根瘤含羞草和土壤中分离的细菌的生物活性化合物的抗生物膜活性：结果：从土壤和植物相关细菌中提取的 8 种上清液对暴力色杆菌具有法定量淬灭活性。所有上清液都对嗜水气单胞菌和哈维弧菌形成的生物膜具有抗生物膜活性。FT5 上清液在破坏（66.59%）和抑制（85.63%）嗜水气单胞菌生物膜方面的活性最高。对于 V. harveyi，PTM3 的上清液显示出最高的破坏活性（72.61%），而RCA7 显示出最高的抑制活性（75.68%）。气相色谱-质谱法（GC-MS）鉴定出脂肪酸、酯和二酮哌嗪是与抗生物膜活性有关的化合物。分子鉴定结果表明，分离物属于芽孢杆菌属、普里斯特菌属和干酪杆菌属。

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来源期刊

BMC Research Notes Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.60

自引率

0.00%

发文量

363

审稿时长

15 weeks

期刊介绍： BMC Research Notes publishes scientifically valid research outputs that cannot be considered as full research or methodology articles. We support the research community across all scientific and clinical disciplines by providing an open access forum for sharing data and useful information; this includes, but is not limited to, updates to previous work, additions to established methods, short publications, null results, research proposals and data management plans.