Structure of Extracellular Vesicles and Their Effect on Bacterial Biofilms Change with the Development of Antibiotic Resistance in the Probiotic Strain Lactiplantibacillus plantarum 8p-a3.
O A Chernova, A R Kayumov, M I Markelova, V V Salnikov, M P Kutyreva, A A Khannanov, M S Fedorova, D E Zhuravleva, N B Baranova, D A Faizullin, Yu F Zuev, V M Chernov
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引用次数: 0
Abstract
Significant changes in lactobacillus-derived extracellular membrane vesicles, which transfer lipids, polysaccharides, proteins, and nucleic acids, were for the first time observed to accompany the development of resistance to antibiotics (amoxicillin and clarithromycin) in vitro in the probiotic strain Lactiplantibacillus plantarum 8p-a3. The changes occur together with large-scale genome rearrangements, changes in the profile of phenotypic sensitivity to antimicrobials of various groups, and evolution of virulence. Changes in vesicles affected their structure, composition, and activity against biofilms of opportunistic bacteria. The data provide for a better understanding of the molecular mechanisms of microbial survival under selective pressure of antimicrobials, the functional potential of probiotic vesicles, and probiotic safety assessments.
在益生菌株植物乳杆菌（Lactiplantibacillus plantarum 8p-a3）的体外抗生素（阿莫西林和克拉霉素）耐药性发展过程中，首次观察到了乳杆菌胞外膜囊泡的显著变化，这些囊泡可转移脂质、多糖、蛋白质和核酸。这些变化与大规模基因组重排、对各类抗菌素的表型敏感性变化以及毒力进化同时发生。囊泡的变化影响了它们的结构、组成和对机会性细菌生物膜的活性。这些数据有助于更好地了解微生物在抗菌素选择性压力下生存的分子机制、益生菌囊泡的功能潜力以及益生菌安全性评估。
期刊介绍：
Doklady Biological Sciences is a journal that publishes new research in biological sciences of great significance. Initially the journal was a forum of the Russian Academy of Science and published only best contributions from Russia in the form of short articles. Now the journal welcomes submissions from any country in the English or Russian language. Every manuscript must be recommended by Russian or foreign members of the Russian Academy of Sciences.
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