Characterization of small RNAs in the spleen of MASH in a non-human primate model

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2024-10-16 DOI:10.1016/j.ygeno.2024.110953

Juan Zhao , Yuelei Zhao , Hongyu Qin , Yun Ye , Liwei Zhang , Ruike Ding , Wenbin Cao , Yanru Zhang , Chenjing Duan , Haoze Leng , Yandong Li , Bo Wang , Liangshuo Hu , Enqi Liu , Pengxiang Qu

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Abstract

Metabolic dysfunction-associated steatotic liver disease (MASLD) and its advanced stage, metabolic dysfunction-associated steatohepatitis (MASH), are increasingly recognized as a global health issue. This study examines the role of small RNAs in the spleen of MASH using a non-human primate model. We performed high-throughput small RNA sequencing on spleen tissues from MASH-primates, revealing significant alterations in the expression of small non-coding RNAs, especially miRNAs. Notably, miR-96, miR-182, miR-183, and miR-122 showed differential expression in MASH spleens. Predictive and validation studies have identified potential target genes, such as PTX3 and NFIX, that were significantly dysregulated in spleens of MASH. These findings characterized small RNAs in spleen of MASH and offer a novel insight for further research for MASH.

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非人灵长类动物模型 MASH 脾脏中小 RNA 的特征。

代谢功能障碍相关性脂肪性肝病（MASLD）及其晚期阶段--代谢功能障碍相关性脂肪性肝炎（MASH）--越来越被认为是一个全球性的健康问题。本研究利用非人灵长类动物模型研究了小 RNA 在 MASH 脾脏中的作用。我们对 MASH-原猴的脾脏组织进行了高通量小 RNA 测序，发现小非编码 RNA，尤其是 miRNA 的表达发生了显著变化。值得注意的是，miR-96、miR-182、miR-183 和 miR-122 在 MASH 脾脏中的表达存在差异。预测和验证研究发现了潜在的靶基因，如 PTX3 和 NFIX，这些基因在 MASH 脾脏中明显失调。这些发现揭示了MASH脾脏中小RNA的特征，为进一步研究MASH提供了新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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