Hypermethylation of the sodium channel beta subunit gene promoter is associated with colorectal cancer.

IF 2.7 3区生物学 Hereditas Pub Date : 2024-10-17 DOI:10.1186/s41065-024-00340-0

Yabin Liu, Ya Duan, Tianliang Bai, Dexian Kong

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Abstract

Aims: To better understand the role of sodium channel beta subunit (SCNN1B) in the initiation and progression of colorectal cancer (CRC) and to identify potential biomarkers for the early detection and prognosis of CRC.

Methods: A total of 74 pairs of CRC tissues and their adjacent normal tissues were collected between October 2016 and November 2017. The methylation levels of the SCNN1B promoter region in CRC tissues and their adjacent normal tissues were investigated by pyrosequencing. The expression of both SCNN1B mRNA and protein were detected by RT‒qPCR and immunohistochemistry, respectively.

Results: The results showed that the methylation levels of the SCNN1B promoter region were significantly higher in CRC tissues than in adjacent normal tissues. The expression levels of SCNN1B mRNA and protein were significantly lower in the CRC tissues than in their adjacent normal tissues. Moreover, Pearson's correlation analysis showed that the methylation levels of the SCNN1B promoter were negatively correlated with the SCNN1B mRNA levels in CRC tissues. In addition, the high methylation levels and low mRNA expression of SCNN1B showed a significant association with advanced tumour stage, increased risk of lymph node metastasis and poor prognosis of CRC patients.

Conclusion: This study suggested that the decreased expression of SCNN1B due to its promoter hypermethylation may play an important role in the progression and prognosis of CRC, and the methylation levels of the SCNN1B promoter may serve as an effective molecular marker for predicting the progression and prognosis of CRC.

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钠通道 beta 亚基基因启动子的高甲基化与结直肠癌有关。

目的：更好地了解钠通道β亚基（SCNN1B）在结直肠癌（CRC）的发生和发展中的作用，并确定CRC早期检测和预后的潜在生物标志物：方法：2016年10月至2017年11月期间，共收集了74对CRC组织及其邻近正常组织。通过热释光测序法研究了CRC组织及其邻近正常组织中SCNN1B启动子区的甲基化水平。分别通过 RT-qPCR 和免疫组化检测 SCNN1B mRNA 和蛋白的表达：结果表明：CRC组织中SCNN1B启动子区域的甲基化水平明显高于邻近的正常组织。CRC 组织中 SCNN1B mRNA 和蛋白的表达水平明显低于邻近的正常组织。此外，Pearson 相关分析表明，CRC 组织中 SCNN1B 启动子的甲基化水平与 SCNN1B mRNA 水平呈负相关。此外，SCNN1B 的高甲基化水平和低 mRNA 表达与 CRC 患者的肿瘤晚期、淋巴结转移风险增加和预后不良有显著相关性：该研究表明，SCNN1B启动子高甲基化导致其表达量下降可能在CRC的进展和预后中发挥重要作用，SCNN1B启动子的甲基化水平可作为预测CRC进展和预后的有效分子标记物。

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来源期刊

Hereditas Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.80

自引率

3.70%

发文量

期刊介绍： For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.