Expression Profile and Prognostic Significance of Pivotal Regulators for N7-Methylguanosine Methylation in Diffuse Large B-Cell Lymphoma.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biotechnology Pub Date : 2024-10-22 DOI:10.1007/s12033-024-01264-w

Cong Wang, Ran Kong, Guangcai Zhong, Peipei Li, Na Wang, Ganyu Feng, Mei Ding, Xiangxiang Zhou

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Abstract

N7-methylguanosine (m7G) occurs by adding a methyl group to the N7 atom of the RNA guanine. Emerging evidence suggests that m7G modification has emerged as a crucial regulator of tumorigenesis, progression, invasion, and metastasis in multiple cancers. Nevertheless, the utility of m7G modification in diffuse large B-cell lymphoma (DLBCL) remains undefined, notably the interaction with the tumor microenvironment (TME). Here, we aimed to identify the expression profile of m7G regulators in DLBCL, construct a novel risk model, and explore their connection with TME. We initially investigated the difference and correlation in m7G regulators' expression in normal and tumor groups, classified patients by consistent clustering analysis, investigated the functional and prognostic significance of the resulting subtypes, and identified prognosis-associated genes by one-way Cox and least absolute shrinkage and selection operator (LASSO) regression calculations, and constructed a risk model. Further analysis showed that correlation among immune cell infiltration with m7G risk score and determined that it impacts the prognosis of DLBCL patients. Our research demonstrated the relevance of m7G regulators to DLBCL prognosis, providing theoretical support for precise prognostic stratification and immunotherapeutic assessment in DLBCL.

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弥漫大 B 细胞淋巴瘤中 N7-甲基鸟苷甲基化关键调节因子的表达谱和预后意义

N7-甲基鸟苷（m7G）是通过在 RNA 鸟嘌呤的 N7 原子上添加一个甲基而发生的。新的证据表明，m7G修饰已成为多种癌症中肿瘤发生、发展、侵袭和转移的关键调节因子。然而，m7G修饰在弥漫大B细胞淋巴瘤（DLBCL）中的作用仍未确定，特别是与肿瘤微环境（TME）的相互作用。在此，我们旨在确定 m7G 调控因子在 DLBCL 中的表达谱，构建一个新的风险模型，并探索它们与 TME 的关系。我们首先研究了正常组和肿瘤组中m7G调控因子表达的差异和相关性，通过一致聚类分析对患者进行了分类，研究了所得亚型的功能和预后意义，并通过单向Cox和最小绝对收缩和选择算子（LASSO）回归计算确定了预后相关基因，构建了风险模型。进一步的分析表明，免疫细胞浸润与m7G风险评分之间存在相关性，并确定其影响DLBCL患者的预后。我们的研究证明了m7G调节因子与DLBCL预后的相关性，为DLBCL的精确预后分层和免疫治疗评估提供了理论支持。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.