Fecal samples and rectal swabs adequately reflect the human colonic luminal microbiota.

IF 12.2 1区 医学 Q1 GASTROENTEROLOGY & HEPATOLOGY Gut Microbes Pub Date : 2024-10-22 DOI:10.1080/19490976.2024.2416912
Julia Rode,Linnea Brengesjö Johnson,Julia König,Ignacio Rangel,Lars Engstrand,Dirk Repsilber,Robert J Brummer
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Abstract

The appropriateness of the fecal microbiota to adequately reflect the gut microbiota composition from more difficult to access luminal content at different colonic locations has been debated. Here, in a healthy population, luminal samples were collected from terminal ileum to rectum using an unique sampling technique without the need of prior bowel cleansing/preparation. Rectal swabs were collected immediately prior colonoscopy by an experienced physician, and fecal samples were collected at home by the participants themselves. Microbiota composition was evaluated as relative abundance, α-diversity and Bray-Curtis dissimilarities. Our data suggest that fecal samples and rectal swabs present noninvasive, easily accessible, low-cost sampling tools that are accurate proxies to characterize luminal large intestinal microbiota composition.
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粪便样本和直肠拭子能充分反映人体结肠腔微生物群。
粪便微生物群是否能充分反映不同结肠位置较难获取的管腔内容物中的肠道微生物群组成一直存在争议。在此,我们采用一种独特的取样技术,从回肠末端到直肠收集健康人群的管腔样本,无需事先进行肠道清洁/准备。直肠拭子由经验丰富的医生在结肠镜检查前立即采集,粪便样本则由参与者在家中自行采集。微生物群组成以相对丰度、α-多样性和布雷-柯蒂斯相似度进行评估。我们的数据表明,粪便样本和直肠拭子是非侵入性、易获取、低成本的采样工具,是描述管腔大肠微生物群组成特征的准确替代物。
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来源期刊
Gut Microbes
Gut Microbes Medicine-Microbiology (medical)
CiteScore
18.20
自引率
3.30%
发文量
196
审稿时长
10 weeks
期刊介绍: The intestinal microbiota plays a crucial role in human physiology, influencing various aspects of health and disease such as nutrition, obesity, brain function, allergic responses, immunity, inflammatory bowel disease, irritable bowel syndrome, cancer development, cardiac disease, liver disease, and more. Gut Microbes serves as a platform for showcasing and discussing state-of-the-art research related to the microorganisms present in the intestine. The journal emphasizes mechanistic and cause-and-effect studies. Additionally, it has a counterpart, Gut Microbes Reports, which places a greater focus on emerging topics and comparative and incremental studies.
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