Photobiomodulation Improves Histological Parameters of Testis and Spermatogenesis in Adult Mice Exposed to Scrotal Hyperthermia in the Prepubertal Phase.

IF 1.4 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING Journal of lasers in medical sciences Pub Date : 2024-09-28 eCollection Date: 2024-01-01 DOI:10.34172/jlms.2024.49
Fakhroddin Aghajanpour, Hojjat-Allah Abbaszadeh, Hamid Nazarian, Azar Afshar, Reza Soltani, Homayoon Bana Derakhshan, Fatemeh Fadaei Fathabadi, Mohsen Norouzian
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Abstract

Introduction: Heat stress is one of the environmental causes of damage to the testis, whose effects are less known before puberty. The aim of the present study was to investigate the impact of photobiomodulation (PBM) on the testis of prepubertal mice subjected to hyperthermia. Methods: Twenty-four three-week-old prepubertal male mice were allocated to the following groups: I) control, II) scrotal hyperthermia (Hyp), and III) Hyp+PBM (n=8/each group). In order to induce hyperthermia, the scrotum was placed in water at 43 °C for 20 minutes every other day for a total duration of 10 days. In the Hyp+PBM group, after hyperthermia induction, the testis of the mice was subjected to laser irradiation at a wavelength of 890 nm (0.03 J/cm2 for 30 seconds) for 35 days. After the mice were sacrificed, the testis and epididymis were removed for testing. Results: Compared with those of the Hyp group, the sperm parameters of the laser irradiation group improved notably. In addition, histological examinations revealed that the final number of testis cells and the volume of tissue in the Hyp+PBM group were dramatically greater than those in the Hyp group. The analysis of molecular data revealed an increase in the expression of mitotic genes and testosterone levels and a decrease in the formation of reactive oxygen species (ROS) and the expression of the apoptotic gene in the testis subjected to PBM. Conclusion: Based on the present findings, laser therapy can reduce complications caused by scrotal hyperthermia during prepuberty and ameliorate spermatogenesis during puberty.

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光生物调节可改善在青春期前暴露于阴囊高热的成年小鼠的睾丸组织学参数和精子发生。
导言热应激是导致睾丸损伤的环境因素之一,但其对青春期前睾丸的影响却鲜为人知。本研究旨在探讨光生物调节(PBM)对青春期前小鼠睾丸的影响。研究方法将 24 只三周大的青春期前雄性小鼠分为以下几组:I)对照组;II)阴囊热疗(Hyp)组;III)Hyp+PBM组(每组8只)。为了诱导高热,每隔一天将阴囊置于 43 °C 的水中 20 分钟,共持续 10 天。在 Hyp+PBM 组中,诱导高热后,对小鼠的睾丸进行波长为 890 纳米的激光照射(0.03 J/cm2,30 秒),持续 35 天。小鼠被处死后,取出睾丸和附睾进行检测。结果与 Hyp 组相比,激光照射组的精子参数明显改善。此外,组织学检查显示,Hyp+PBM 组最终的睾丸细胞数量和组织体积均显著大于 Hyp 组。分子数据分析显示,接受 PBM 治疗的睾丸中,有丝分裂基因和睾酮水平的表达增加,活性氧(ROS)的形成和凋亡基因的表达减少。结论根据目前的研究结果,激光疗法可以减少青春期前阴囊高热引起的并发症,并改善青春期的精子发生。
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来源期刊
Journal of lasers in medical sciences
Journal of lasers in medical sciences RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
3.00
自引率
13.30%
发文量
24
期刊介绍: The "Journal of Lasers in Medical Sciences " is a scientific quarterly publication of the Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences. This journal received a scientific and research rank from the national medical publication committee. This Journal accepts original papers, review articles, case reports, brief reports, case series, photo assays, letters to the editor, and commentaries in the field of laser, or light in any fields of medicine such as the following medical specialties: -Dermatology -General and Vascular Surgery -Oncology -Cardiology -Dentistry -Urology -Rehabilitation -Ophthalmology -Otorhinolaryngology -Gynecology & Obstetrics -Internal Medicine -Orthopedics -Neurosurgery -Radiology -Pain Medicine (Algology) -Basic Sciences (Stem cell, Cellular and Molecular application and physic)
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