Safety Assessment and Evaluation of Probiotic Potential of Lactobacillus bulgaricus IDCC 3601 for Human Use.

IF 4.1 2区生物学 Q2 MICROBIOLOGY Microorganisms Pub Date : 2024-10-15 DOI:10.3390/microorganisms12102063

Minjee Lee, Won-Yeong Bang, Han-Bin Lee, Soo-Yeon Yang, Kyu-Shik Lee, Hae-Ji Kang, Sun-Mee Hong, Jungwoo Yang

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Abstract

Lactic acid bacteria (LAB) are probiotic microorganisms widely used for their health benefits in the food industry. However, recent concerns regarding their safety have highlighted the need for comprehensive safety assessments. In this study, we aimed to evaluate the safety of L. bulgaricus IDCC 3601, isolated from homemade plain yogurt, via genomic, phenotypic, and toxicity-based analyses. L. bulgaricus IDCC 3601 possessed a single circular chromosome of 1,865,001 bp, with a GC content of 49.72%, and 1910 predicted coding sequences. No virulence or antibiotic resistance genes were detected. Although L. bulgaricus IDCC 3601 exhibited antibiotic resistance to gentamicin and kanamycin, this resistance is an intrinsic feature of this species. L. bulgaricus IDCC 3601 did not produce biogenic amines and did not exhibit hemolytic activity. Phenotypic analysis of enzyme activity and carbohydrate fermentation profiles revealed the metabolic features of L. bulgaricus IDCC 3601. Moreover, no deaths or abnormalities were observed in single-dose oral toxicity tests, suggesting that L. bulgaricus IDCC 3601 has no adverse effect on human health. Finally, L. bulgaricus IDCC 3601 inhibited the growth of potential carbapenem-resistant Enterobacteriaceae. Therefore, our results suggest that L. bulgaricus IDCC 3601 is a safe probiotic strain for human consumption.

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保加利亚乳杆菌 IDCC 3601 用于人类的安全性评估和益生菌潜力评价。

乳酸菌（LAB）是一种益生微生物，因其对健康有益而被广泛用于食品工业。然而，最近人们对其安全性的担忧凸显了对其进行全面安全评估的必要性。在本研究中，我们旨在通过基因组、表型和毒性分析，评估从自制原味酸奶中分离出来的保加利亚乳杆菌 IDCC 3601 的安全性。保加利亚乳杆菌 IDCC 3601 拥有 1,865,001 bp 的单环染色体，GC 含量为 49.72%，1910 个预测编码序列。没有检测到毒力基因或抗生素耐药性基因。虽然保加利亚杆菌 IDCC 3601 对庆大霉素和卡那霉素具有抗药性，但这种抗药性是该菌种的固有特征。保加利亚乳杆菌 IDCC 3601 不产生生物胺，也不表现出溶血活性。酶活性和碳水化合物发酵概况的表型分析表明了保加利亚乳杆菌 IDCC 3601 的代谢特征。此外，在单剂量口服毒性试验中未发现死亡或异常情况，这表明保加利亚乳杆菌 IDCC 3601 不会对人体健康产生不利影响。最后，保加利亚乳杆菌 IDCC 3601 还能抑制潜在的耐碳青霉烯类肠杆菌的生长。因此，我们的研究结果表明保加利亚乳杆菌 IDCC 3601 是一种可供人类食用的安全益生菌株。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.