Aflatoxins Levels in Concentrate Feeds Collected from Specialized Dairy Farms and Local Markets in Selected Urban Centers of Eastern Ethiopia.

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2024-09-27 DOI:10.3390/toxins16100418
Angassa Tesfaye, Mohammed Yusuf Kurtu, Yesihak Yusuf Mummed, Abdi Mohammed
{"title":"Aflatoxins Levels in Concentrate Feeds Collected from Specialized Dairy Farms and Local Markets in Selected Urban Centers of Eastern Ethiopia.","authors":"Angassa Tesfaye, Mohammed Yusuf Kurtu, Yesihak Yusuf Mummed, Abdi Mohammed","doi":"10.3390/toxins16100418","DOIUrl":null,"url":null,"abstract":"<p><p>Aflatoxin constitutes a significant concern for food and feed safety, posing detrimental health risks to both animals and humans. This study aimed to examine the prevalence and concentration of aflatoxins in maize feed, total mixed ration, and wheat bran collected from specialized dairy farms and local markets in three major urban centers in eastern Ethiopia. A total of 180 feed samples were collected from September 2021 to January 2022 in Chiro town, Dire Dawa city, and Harar city. These samples underwent thorough extraction and immunoaffinity clean-up before aflatoxin analysis using HPLC/FLD. The results revealed that AFB<sub>1</sub>, AFB<sub>2</sub>, AFG<sub>1</sub>, AFG<sub>2</sub>, and TAF contamination was detected in 72.2%, 66.1%, 71.1%, 68.7%, and 82.8% of the feed samples, respectively. The corresponding mean levels of each aflatoxin were 28.15 ± 3.50, 3.3 ± 0.40, 19.87 ± 1.87, 2.7 ± 0.32, and 54.01 ± 4.72 µg/kg, respectively. The occurrence and levels of aflatoxin varied across different study sites and feed types. Notably, feeds from Dire Dawa city exhibited significantly higher mean levels of AFB<sub>1</sub> (43.98 ± 5.3 µg/kg), AFB<sub>2</sub> (5.69 ± 0.6 µg/kg), AFG<sub>1</sub> (32.25 ± 2.7 µg/kg), and AFG<sub>2</sub> (5.01 ± 0.5 µg/kg) than feeds from other urban centers did. Additionally, a significantly higher occurrence of AFB<sub>1</sub> (29.4%) and AFG<sub>1</sub> (28.3%) was detected in feed from Dire Dawa city. Similarly, the total mixed ration (TMR) displayed significantly higher levels of AFB<sub>1</sub> (50.67 ± 5.2 µg/kg), AFB<sub>2</sub> (4.74 ± 0.6 µg/kg), AFG<sub>1</sub> (32.87 ± 2.6 µg/kg), and AFG<sub>2</sub> (3.86 ± 0.5 µg/kg) compared to the other feed types. Moreover, a significantly higher occurrence of AFB<sub>1</sub> (30.7%) and AFG<sub>1</sub> (28.7%) was detected in the TMR. Furthermore, a moderate correlation was observed between the count of aflatoxigenic <i>Aspergillus</i> species and the levels of TAF in the feed samples. Overall, this study underscores the widespread presence of aflatoxin contamination in dairy feeds in eastern Ethiopia, highlighting the urgent need for stringent monitoring and mitigation measures to ensure food and feed safety, as well as public health.</p>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511069/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/toxins16100418","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0

Abstract

Aflatoxin constitutes a significant concern for food and feed safety, posing detrimental health risks to both animals and humans. This study aimed to examine the prevalence and concentration of aflatoxins in maize feed, total mixed ration, and wheat bran collected from specialized dairy farms and local markets in three major urban centers in eastern Ethiopia. A total of 180 feed samples were collected from September 2021 to January 2022 in Chiro town, Dire Dawa city, and Harar city. These samples underwent thorough extraction and immunoaffinity clean-up before aflatoxin analysis using HPLC/FLD. The results revealed that AFB1, AFB2, AFG1, AFG2, and TAF contamination was detected in 72.2%, 66.1%, 71.1%, 68.7%, and 82.8% of the feed samples, respectively. The corresponding mean levels of each aflatoxin were 28.15 ± 3.50, 3.3 ± 0.40, 19.87 ± 1.87, 2.7 ± 0.32, and 54.01 ± 4.72 µg/kg, respectively. The occurrence and levels of aflatoxin varied across different study sites and feed types. Notably, feeds from Dire Dawa city exhibited significantly higher mean levels of AFB1 (43.98 ± 5.3 µg/kg), AFB2 (5.69 ± 0.6 µg/kg), AFG1 (32.25 ± 2.7 µg/kg), and AFG2 (5.01 ± 0.5 µg/kg) than feeds from other urban centers did. Additionally, a significantly higher occurrence of AFB1 (29.4%) and AFG1 (28.3%) was detected in feed from Dire Dawa city. Similarly, the total mixed ration (TMR) displayed significantly higher levels of AFB1 (50.67 ± 5.2 µg/kg), AFB2 (4.74 ± 0.6 µg/kg), AFG1 (32.87 ± 2.6 µg/kg), and AFG2 (3.86 ± 0.5 µg/kg) compared to the other feed types. Moreover, a significantly higher occurrence of AFB1 (30.7%) and AFG1 (28.7%) was detected in the TMR. Furthermore, a moderate correlation was observed between the count of aflatoxigenic Aspergillus species and the levels of TAF in the feed samples. Overall, this study underscores the widespread presence of aflatoxin contamination in dairy feeds in eastern Ethiopia, highlighting the urgent need for stringent monitoring and mitigation measures to ensure food and feed safety, as well as public health.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
从埃塞俄比亚东部部分城市中心的专业奶牛场和当地市场采集的浓缩饲料中的黄曲霉毒素含量。
黄曲霉毒素是食品和饲料安全方面的一个重大问题,对动物和人类的健康都有不利影响。本研究旨在检测从埃塞俄比亚东部三个主要城市中心的专业奶牛场和当地市场收集的玉米饲料、全混日粮和麦麸中黄曲霉毒素的流行率和浓度。2021 年 9 月至 2022 年 1 月期间,在奇罗镇、德雷达瓦市和哈拉尔市共采集了 180 份饲料样品。在使用高效液相色谱/荧光法分析黄曲霉毒素之前,对这些样品进行了彻底提取和免疫亲和净化。结果显示,在 72.2%、66.1%、71.1%、68.7% 和 82.8% 的饲料样品中分别检测到 AFB1、AFB2、AFG1、AFG2 和 TAF 污染。各种黄曲霉毒素的平均含量分别为每公斤 28.15 ± 3.50 微克、3.3 ± 0.40 微克、19.87 ± 1.87 微克、2.7 ± 0.32 微克和 54.01 ± 4.72 微克。黄曲霉毒素的发生率和含量在不同的研究地点和饲料类型之间存在差异。值得注意的是,迪雷达瓦市饲料中的 AFB1(43.98 ± 5.3 µg/kg)、AFB2(5.69 ± 0.6 µg/kg)、AFG1(32.25 ± 2.7 µg/kg)和 AFG2(5.01 ± 0.5 µg/kg)的平均含量明显高于其他城市中心的饲料。此外,在迪雷达瓦市的饲料中检测到的 AFB1(29.4%)和 AFG1(28.3%)含量也明显较高。同样,与其他类型的饲料相比,全混合饲料(TMR)中的 AFB1(50.67 ± 5.2 µg/kg)、AFB2(4.74 ± 0.6 µg/kg)、AFG1(32.87 ± 2.6 µg/kg)和 AFG2(3.86 ± 0.5 µg/kg)含量也明显较高。此外,在 TMR 中检测到的 AFB1(30.7%)和 AFG1(28.7%)含量明显更高。此外,还观察到饲料样本中黄曲霉菌的数量与 TAF 含量之间存在一定的相关性。总之,这项研究强调了埃塞俄比亚东部地区奶牛饲料中黄曲霉毒素污染的广泛存在,突出表明迫切需要采取严格的监测和缓解措施,以确保食品和饲料安全以及公众健康。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
期刊最新文献
Hyperbaric oxygen treatment promotes tendon-bone interface healing in a rabbit model of rotator cuff tears. Oxygen-ozone therapy for myocardial ischemic stroke and cardiovascular disorders. Comparative study on the anti-inflammatory and protective effects of different oxygen therapy regimens on lipopolysaccharide-induced acute lung injury in mice. Heme oxygenase/carbon monoxide system and development of the heart. Hyperbaric oxygen for moderate-to-severe traumatic brain injury: outcomes 5-8 years after injury.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1