Deciphering the Genetic Architecture of Staphylococcus warneri Prophage vB_G30_01: A Comprehensive Molecular Analysis.

IF 3.8 3区医学 Q2 VIROLOGY Viruses-Basel Pub Date : 2024-10-19 DOI:10.3390/v16101631

Fangxiong Pu, Ning Zhang, Jiahe Pang, Nan Zeng, Faryal Babar Baloch, Zijing Li, Bingxue Li

{"title":"Deciphering the Genetic Architecture of Staphylococcus warneri Prophage vB_G30_01: A Comprehensive Molecular Analysis.","authors":"Fangxiong Pu, Ning Zhang, Jiahe Pang, Nan Zeng, Faryal Babar Baloch, Zijing Li, Bingxue Li","doi":"10.3390/v16101631","DOIUrl":null,"url":null,"abstract":"The current knowledge of Staphylococcus warneri phages is limited, with few genomes sequenced and characterized. In this study, a prophage, vB_G30_01, isolated from Staphylococcus warneri G30 was characterized and evaluated for its lysogenic host range. The phage was studied using transmission electron microscopy and a host range. The phage genome was sequenced and characterized in depth, including phylogenetic and taxonomic analyses. The linear dsDNA genome of vB_G30_01 contains 67 predicted open reading frames (ORFs), classifying it within Bronfenbrennervirinae. With a total of 10 ORFs involved in DNA replication-related and transcriptional regulator functions, vB_G30_01 may play a role in the genetics and transcription of a host. Additionally, vB_G30_01 possesses a complete set of genes related to host lysogeny and lysis, implying that vB_G30_01 may influence the survival and adaptation of its host. Furthermore, a comparative genomic analysis reveals that vB_G30_01 shares high genomic similarity with other Staphylococcus phages and is relatively closely related to those of Exiguobacterium and Bacillus, which, in combination with the cross-infection assay, suggests possible cross-species infection capabilities. This study enhances the understanding of Staphylococcus warneri prophages, providing insights into phage-host interactions and potential horizontal gene transfer.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":"16 10","pages":""},"PeriodicalIF":3.8000,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512304/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Viruses-Basel","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/v16101631","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VIROLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The current knowledge of Staphylococcus warneri phages is limited, with few genomes sequenced and characterized. In this study, a prophage, vB_G30_01, isolated from Staphylococcus warneri G30 was characterized and evaluated for its lysogenic host range. The phage was studied using transmission electron microscopy and a host range. The phage genome was sequenced and characterized in depth, including phylogenetic and taxonomic analyses. The linear dsDNA genome of vB_G30_01 contains 67 predicted open reading frames (ORFs), classifying it within Bronfenbrennervirinae. With a total of 10 ORFs involved in DNA replication-related and transcriptional regulator functions, vB_G30_01 may play a role in the genetics and transcription of a host. Additionally, vB_G30_01 possesses a complete set of genes related to host lysogeny and lysis, implying that vB_G30_01 may influence the survival and adaptation of its host. Furthermore, a comparative genomic analysis reveals that vB_G30_01 shares high genomic similarity with other Staphylococcus phages and is relatively closely related to those of Exiguobacterium and Bacillus, which, in combination with the cross-infection assay, suggests possible cross-species infection capabilities. This study enhances the understanding of Staphylococcus warneri prophages, providing insights into phage-host interactions and potential horizontal gene transfer.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

解密华氏葡萄球菌噬菌体 vB_G30_01 的基因结构：综合分子分析。

目前对沃纳葡萄球菌噬菌体的了解还很有限，很少有基因组被测序和鉴定。本研究对从沃那葡萄球菌 G30 中分离出的噬菌体 vB_G30_01 进行了鉴定，并评估了其溶解性宿主范围。利用透射电子显微镜和宿主范围对噬菌体进行了研究。对噬菌体基因组进行了深入测序和特征描述，包括系统发生学和分类学分析。vB_G30_01 的线性 dsDNA 基因组包含 67 个预测的开放阅读框（ORF），可将其归入 Bronfenbrennervirinae。vB_G30_01共有10个ORF参与DNA复制相关和转录调节功能，可能在宿主的遗传和转录中发挥作用。此外，vB_G30_01 还拥有一整套与宿主溶解和裂解有关的基因，这意味着 vB_G30_01 可能会影响宿主的生存和适应。此外，基因组比较分析表明，vB_G30_01 与其他葡萄球菌噬菌体的基因组相似度很高，与外植体和芽孢杆菌的基因组相似度相对较高，结合交叉感染试验，表明其可能具有跨物种感染能力。这项研究加深了人们对沃纳里葡萄球菌噬菌体的了解，为噬菌体与宿主的相互作用和潜在的水平基因转移提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Viruses-Basel VIROLOGY-

CiteScore

7.30

自引率

12.80%

发文量

2445

审稿时长

1 months

期刊介绍： Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.