Altered Endometrial Microbiota Profile Is Associated With Poor Endometrial Receptivity of Repeated Implantation Failure

IF 2.5 3区 医学 Q3 IMMUNOLOGY American Journal of Reproductive Immunology Pub Date : 2024-10-29 DOI:10.1111/aji.70005
Rongxue Zhang, Miaomiao Wang, Jixiang Zhong, Huiying Xue
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Abstract

Purpose

To gain insight into the endometrial pathophysiology of unexplained repeated implantation failure (RIF), we examined the characteristics of genital tract microbiota and explored the correlation between the microbiota and endometrial receptivity.

Methods

Vaginal secretion (VS) and endometrial biopsy (EB) samples were collected from patients with RIF (RIF group, n = 32) and those with infertility who had achieved pregnancy during their initial embryo transfer cycle (control group, n = 18). 16S ribosomal RNA sequencing and quantitative PCR were performed to characterize the microbiota of the two groups. Spearman's correlation analysis was performed to determine the relationship between endometrial receptivity markers and endometrial microbiota.

Results

Endometrial microbiota exhibited distinct characteristics from vaginal microbiota, with a higher alpha-diversity. Alpha-diversity of the endometrial microbiota was higher in the RIF group than in the control group. Compared with the control group, the RIF group had a significant decrease in endometrial Lactobacillus abundance and an increase in Gardnerella and Acinetobacter abundances. The expression levels of endometrial receptivity markers, including homeobox A11, integrin αvβ3, leukemia inhibitor factor, matrix metalloproteinase-9, and vascular endothelial growth factor, were lower in the RIF group than in the control group. Moreover, the expression levels of these markers were correlated with endometrial Lactobacillus, Gardnerella, and Acinetobacter abundances.

Conclusion

RIF is characterized by endometrial microbiota dysbiosis and poor endometrial receptivity. Moreover, abnormal endometrial microbiota is associated with impaired endometrial receptivity, which may be a potential cause of unexplained RIF.

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子宫内膜微生物群谱系改变与反复种植失败的子宫内膜接受能力差有关
目的:为了深入了解原因不明的反复植入失败(RIF)的子宫内膜病理生理学,我们研究了生殖道微生物群的特征,并探讨了微生物群与子宫内膜接受性之间的相关性:阴道分泌物(VS)和子宫内膜活检(EB)样本分别取自RIF患者(RIF组,n = 32)和在首次胚胎移植周期中妊娠的不孕症患者(对照组,n = 18)。通过 16S 核糖体 RNA 测序和定量 PCR 分析两组患者的微生物群特征。进行斯皮尔曼相关性分析以确定子宫内膜受孕标志物与子宫内膜微生物群之间的关系:结果:子宫内膜微生物群表现出与阴道微生物群不同的特征,α-多样性更高。RIF 组子宫内膜微生物群的α-多样性高于对照组。与对照组相比,RIF 组的子宫内膜乳酸杆菌数量显著减少,而加德纳菌和醋氨曲霉菌数量增加。RIF组子宫内膜接受性标志物的表达水平低于对照组,包括同种异体A11、整合素αvβ3、白血病抑制因子、基质金属蛋白酶-9和血管内皮生长因子。此外,这些标记物的表达水平与子宫内膜乳酸杆菌、加德纳菌和醋酐菌的数量相关:结论:RIF 的特点是子宫内膜微生物群失调和子宫内膜接受能力差。结论:RIF 的特点是子宫内膜微生物群失调和子宫内膜接受能力差,而且子宫内膜微生物群异常与子宫内膜接受能力受损有关,这可能是导致原因不明的 RIF 的潜在原因。
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来源期刊
CiteScore
6.20
自引率
5.60%
发文量
314
审稿时长
2 months
期刊介绍: The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.
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