Functional analysis of TkWRKY33: A key regulator in drought-induced natural rubber synthesis in Taraxacum kok-saghyz

IF 6.1 2区生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2024-10-25 DOI:10.1016/j.plaphy.2024.109232

Yulin Wu , Yaxin Liu , Yunchuan Zhang , Gaoquan Dong , Jie Yan , Hao Zhang

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Abstract

WRKY proteins, which form a transcription factor superfamily that responds to jasmonic acid (JA) signals, regulate various developmental processes and stress responses in plants, including Taraxacum kok-saghyz (TKS). TKS serves as an ideal model plant for studying rubber production and lays the foundation for a comprehensive understanding of JA-mediated regulation of natural rubber synthesis. In the present study, we screened and identified a valuable transcription factor, TkWRKY33, based on transcriptome data from TKS in response to JA. We investigated its role in the regulation of natural rubber synthesis within the JA signaling pathway and its function in response to drought stress. Through protein-protein interactions and transcriptional regulation analysis, we found that TkWRKY33 may regulate natural rubber synthesis through the JA-TkMPK3-TkWRKY33-(TkGGPS5/TkACAT8) cascade pathway, possibly by participating in JA-activated mitogen-activated protein kinase (MAPK) signaling. Overexpression of TkWRKY33 in tobacco, along with functional analysis of drought resistance and comparative analysis of natural rubber content after drought stress, revealed that TkWRKY33 not only enhances plant drought resistance by regulating the expression of genes related to reactive oxygen species (ROS) scavenging through the JA signaling pathway, but also has a close relationship with the signal transduction pathway mediated by the JA hormone in regulating natural rubber synthesis.

The TkWRKY33 is recognized as a valuable transcription factor, which likely plays a role in regulating natural rubber biosynthesis through the JA-activated MAPK cascade signaling pathway JA-TkMPK3-TkWRKY33-(TkGGPS5/TkACAT8).

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TkWRKY33 的功能分析：干旱诱导 Taraxacum kok-saghyz 天然橡胶合成的关键调节因子。

WRKY 蛋白是对茉莉酸（JA）信号做出响应的转录因子超家族，它调控植物的各种发育过程和胁迫响应，包括蒲公英（Taraxacum kok-saghyz，TKS）。TKS 是研究橡胶生产的理想模式植物，为全面了解 JA 介导的天然橡胶合成调控奠定了基础。在本研究中，我们根据 TKS 对 JA 的响应转录组数据筛选并鉴定了一个有价值的转录因子 TkWRKY33。我们研究了它在 JA 信号通路中调控天然橡胶合成的作用及其对干旱胁迫的响应功能。通过蛋白-蛋白相互作用和转录调控分析，我们发现 TkWRKY33 可能通过 JA-TkMPK3-TkWRKY33-(TkGGPS5/TkACAT8) 级联途径调控天然橡胶的合成，可能是通过参与 JA 激活的丝裂原活化蛋白激酶（MAPK）信号传导。在烟草中过表达 TkWRKY33 并进行抗旱性功能分析和干旱胁迫后天然橡胶含量的比较分析表明，TkWRKY33 不仅通过 JA 信号通路调节活性氧清除相关基因的表达来增强植物的抗旱性，而且与 JA 激素介导的信号转导通路在调节天然橡胶合成方面有着密切的关系。TkWRKY33 被认为是一种重要的转录因子，它很可能通过 JA 激活的 MAPK 级联信号通路 JA-TkMPK3-TkWRKY33-(TkGGPS5/TkACAT8) 在调节天然橡胶生物合成中发挥作用。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.