Low-intensity pulsed ultrasound reduces oxidative and endoplasmic reticulum stress in motor neuron cells

IF 3.8 2区 物理与天体物理 Q1 ACOUSTICS Ultrasonics Pub Date : 2024-10-28 DOI:10.1016/j.ultras.2024.107499
Thi-Thuyet Truong , Chih-Chung Huang , Wen-Tai Chiu
{"title":"Low-intensity pulsed ultrasound reduces oxidative and endoplasmic reticulum stress in motor neuron cells","authors":"Thi-Thuyet Truong ,&nbsp;Chih-Chung Huang ,&nbsp;Wen-Tai Chiu","doi":"10.1016/j.ultras.2024.107499","DOIUrl":null,"url":null,"abstract":"<div><div>Endoplasmic reticulum (ER) stress is associated with oxidative stress, which is integral to the development of various pathological conditions, including neurodegenerative disorders. In this study, using NSC-34-a hybrid cell line established by fusing motor neuron–rich embryonic spinal cord cells with mouse neuroblastoma cells-we investigated the effects of low-intensity pulsed ultrasound (LIPUS) stimulation on oxidative (reactive oxygen species)/ER stress-induced neurodegeneration. An ultrasound transducer with a center frequency of 1.15 MHz and a spatial peak temporal average intensity of 357 mW/cm<sup>2</sup> was used for delivering ultrasound (for 8 min, via a water-filled tube) to motor neuron cells seeded in a plastic culture dish. LIPUS stimulation significantly increased the level of the antiapoptotic protein B-cell lymphoma 2 (BCL-2) and inhibited the expression of apoptosis-associated proteins such as BCL-2-associated X protein (BAX), CCAAT/enhancer-binding protein-homologous protein (CHOP), and caspase-12, thus extending the survival of motor neurons. LIPUS stimulation also enhanced Ca<sup>2+</sup> signaling and activated the Ca<sup>2+</sup>-dependent transcription factors as nuclear factor of activated T cells (NFAT) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Furthermore, LIPUS stimulation induced the activation of the serine/threonine kinase protein kinase B (AKT). Thus, LIPUS stimulation prevented oxidative/ER stress–mediated mitochondrial dysfunction. In conclusion, as a safe and noninvasive method, LIPUS stimulation can facilitate further development of ultrasound neuromodulation as a tool for neuroscience research.</div></div>","PeriodicalId":23522,"journal":{"name":"Ultrasonics","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ultrasonics","FirstCategoryId":"101","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0041624X24002622","RegionNum":2,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ACOUSTICS","Score":null,"Total":0}
引用次数: 0

Abstract

Endoplasmic reticulum (ER) stress is associated with oxidative stress, which is integral to the development of various pathological conditions, including neurodegenerative disorders. In this study, using NSC-34-a hybrid cell line established by fusing motor neuron–rich embryonic spinal cord cells with mouse neuroblastoma cells-we investigated the effects of low-intensity pulsed ultrasound (LIPUS) stimulation on oxidative (reactive oxygen species)/ER stress-induced neurodegeneration. An ultrasound transducer with a center frequency of 1.15 MHz and a spatial peak temporal average intensity of 357 mW/cm2 was used for delivering ultrasound (for 8 min, via a water-filled tube) to motor neuron cells seeded in a plastic culture dish. LIPUS stimulation significantly increased the level of the antiapoptotic protein B-cell lymphoma 2 (BCL-2) and inhibited the expression of apoptosis-associated proteins such as BCL-2-associated X protein (BAX), CCAAT/enhancer-binding protein-homologous protein (CHOP), and caspase-12, thus extending the survival of motor neurons. LIPUS stimulation also enhanced Ca2+ signaling and activated the Ca2+-dependent transcription factors as nuclear factor of activated T cells (NFAT) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Furthermore, LIPUS stimulation induced the activation of the serine/threonine kinase protein kinase B (AKT). Thus, LIPUS stimulation prevented oxidative/ER stress–mediated mitochondrial dysfunction. In conclusion, as a safe and noninvasive method, LIPUS stimulation can facilitate further development of ultrasound neuromodulation as a tool for neuroscience research.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
低强度脉冲超声可降低运动神经细胞的氧化应激和内质网应激。
内质网(ER)应激与氧化应激有关,而氧化应激与包括神经退行性疾病在内的各种病理状况的发展密不可分。本研究利用NSC-34--一种由富含运动神经元的胚胎脊髓细胞与小鼠神经母细胞瘤细胞融合而成的杂交细胞系--研究了低强度脉冲超声(LIPUS)刺激对氧化(活性氧)/ER应激诱导的神经退行性病变的影响。使用中心频率为 1.15 MHz、空间峰值时间平均强度为 357 mW/cm2 的超声换能器向塑料培养皿中播种的运动神经元细胞发射超声波(持续 8 分钟,通过注水管)。LIPUS刺激能明显提高抗凋亡蛋白B细胞淋巴瘤2(BCL-2)的水平,抑制BCL-2相关X蛋白(BAX)、CCAAT/增强子结合蛋白同源蛋白(CHOP)和caspase-12等凋亡相关蛋白的表达,从而延长运动神经元的存活时间。LIPUS 刺激还增强了 Ca2+ 信号传导,激活了 Ca2+ 依赖性转录因子,如活化 T 细胞核因子(NFAT)和活化 B 细胞核因子卡巴轻链增强因子(NF-κB)。此外,LIPUS 还能诱导丝氨酸/苏氨酸激酶蛋白激酶 B(AKT)的活化。因此,刺激 LIPUS 可防止氧化/ER 应激介导的线粒体功能障碍。总之,作为一种安全无创的方法,LIPUS 刺激可促进超声神经调控作为神经科学研究工具的进一步发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Ultrasonics
Ultrasonics 医学-核医学
CiteScore
7.60
自引率
19.00%
发文量
186
审稿时长
3.9 months
期刊介绍: Ultrasonics is the only internationally established journal which covers the entire field of ultrasound research and technology and all its many applications. Ultrasonics contains a variety of sections to keep readers fully informed and up-to-date on the whole spectrum of research and development throughout the world. Ultrasonics publishes papers of exceptional quality and of relevance to both academia and industry. Manuscripts in which ultrasonics is a central issue and not simply an incidental tool or minor issue, are welcomed. As well as top quality original research papers and review articles by world renowned experts, Ultrasonics also regularly features short communications, a calendar of forthcoming events and special issues dedicated to topical subjects.
期刊最新文献
Band edge modulation for high-performance LL-SAW resonators on LiNbO3/SiC by introducing an ultra-thin intermediate oxide layer. Low-intensity pulsed ultrasound reduces oxidative and endoplasmic reticulum stress in motor neuron cells RFImageNet framework for segmentation of ultrasound images with spectra-augmented radiofrequency signals Ultrasonic backscattering measurement of hardness gradient distribution in polycrystalline materials Simulation and experimentation of nonlinear Rayleigh wave inspection of fatigue surface microcracks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1