Red fluorescent AIE bioprobes with a large Stokes shift for droplet-specific imaging and fatty liver diagnosis

IF 4.3 2区化学 Q1 SPECTROSCOPY Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2024-10-23 DOI:10.1016/j.saa.2024.125325

Yu-Ting Cai , Yan-Chao Liu , Ying-Ying Gu , Ya-Qi Zhu , Yong-Hong Liu , Jing Chen , Yuan Yang , Ming-Xuan Liu

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Abstract

Lipid droplets (LDs) as spherical dynamic subcellular organelles, play an important role in various cellular functions such as protein degradation, lipid metabolism, energy storage, signal transduction, and membrane formation. Abnormal function of LDs will lead to a series of diseases and hence monitoring the status of LDs is particularly important. In this study, we synthesized a water-insoluble red fluorescent emitting small molecule fluorescent probe (TPE-TCF), which exhibited aggregation-induced emission (AIE) properties and enabled highly selective real-time imaging of LDs (Pearson’s R value was 0.90). More interestingly, this probe was able to track the dynamic processes of LDs in living cells, including lipophagy, and monitor fatty liver disease in mice. Therefore, TPE-TCF with red fluorescence emission, good biocompatibility, large Stokes shift, AIE properties, LDs imaging, and fatty liver recognition capabilities can be practically used in more LDs-related diseases.

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用于液滴特异性成像和脂肪肝诊断的具有较大斯托克斯位移的红色荧光 AIE 生物探针

脂滴（LDs）作为球形动态亚细胞器，在蛋白质降解、脂质代谢、能量储存、信号转导和膜形成等多种细胞功能中发挥着重要作用。LDs 功能异常会导致一系列疾病，因此监测 LDs 的状态尤为重要。在这项研究中，我们合成了一种水不溶性红色荧光发射小分子荧光探针（TPE-TCF），它具有聚集诱导发射（AIE）特性，能对 LDs 进行高选择性实时成像（皮尔逊 R 值为 0.90）。更有趣的是，这种探针能够跟踪活细胞中低密度脂蛋白的动态过程，包括噬脂过程，并监测小鼠的脂肪肝。因此，TPE-TCF 具有红色荧光发射、良好的生物相容性、较大的斯托克斯偏移、AIE 特性、LDs 成像和脂肪肝识别能力，可实际应用于更多与 LDs 相关的疾病。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.