Application of Polydopamine-Based Magnetic Solid-Phase Extraction for Highly Sensitive Determination of Aristolochic Acid I from Traditional Chinese Medicine Samples.

IF 1.5 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of chromatographic science Pub Date : 2024-10-29 DOI:10.1093/chromsci/bmae055

Huimin Li, Huina Cao, Zixin Zhang, Fei Tian, Yi Zhang, Lijie Wu

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Abstract

A low cost-effective and simple synthesis method combining magnetic solid-phase extraction (MSPE) and high-pressure liquid chromatography was developed for the analysis of aristolochic acids I (AAI) in traditional Chinese medicine samples. A novel polydopamine (PDA) modified magnetic nanoparticles with one single carbon layer (Fe3O4@1C NPs) via one-pot hydrothermal approach was prepared and then successfully employed to extract AAI for the first time. Dopamine (DA) can form a PDA layer on Fe3O4@1C NPs surface through self-polymerization to form Fe3O4@1C@PDA. As a surface modifier of DA, PDA offered more adsorption sites to AAI due to π-π stacking, hydrogen bonding and electrostatic interactions. The parameters of MSPE were optimized by univariate and multivariate methods (Box-Behnken design) in detail. High degree of linearity was obtained in the range of 0.05-200.0 μg/mL. The limits of detection (S/N = 3) and quantification (S/N = 10) were 0.08 and 0.25 μg/mL, respectively. The recoveries of AAI in spiked Xiaoqinglong mixture samples were in the range of 86.7 to 108.5% with the relative standard deviation of less than 5.2%. Thus, a fast, convenient, sensitive and eco-friendly method was successfully proposed and became a promising approach for the determination of AAI in herbal plants or its preparation in the manufacturing procedure.

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应用聚多巴胺磁性固相萃取技术高灵敏测定中药样品中的马兜铃酸 I

为分析中药样品中的马兜铃酸I（AAI），建立了一种低成本、简便的磁性固相萃取（MSPE）与高压液相色谱相结合的合成方法。该方法通过一锅水热法制备了具有单碳层的新型聚多巴胺（PDA）修饰磁性纳米粒子（Fe3O4@1C NPs），并首次成功应用于AAI的提取。多巴胺（DA）可通过自聚合作用在 Fe3O4@1C NPs 表面形成 PDA 层，形成 Fe3O4@1C@PDA。作为DA的表面改性剂，PDA通过π-π堆积、氢键和静电作用为AAI提供了更多的吸附位点。通过单变量和多变量方法（Box-Behnken 设计）对 MSPE 的参数进行了详细优化。MSPE 在 0.05-200.0 μg/mL 范围内线性关系良好。检出限（S/N = 3）和定量限（S/N = 10）分别为 0.08 和 0.25 μg/mL。小青龙混合物样品中AAI的加标回收率为86.7%～108.5%，相对标准偏差小于5.2%。该方法快速、简便、灵敏、环保，可用于中药材中AAI含量的检测或生产工艺中AAI的制备。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.