The Quantitative In Vivo Assessment of Diabetic and Non-Diabetic Skin Wound Healing Using Phasor-FLIM Approach.

Journal of biophotonics Pub Date : 2024-10-29 DOI:10.1002/jbio.202400227

Hala Zuhayri, Tatiana B Lepekhina, Viktor V Nikolaev, Houssain Baalbaki, Anastasia I Knyazkova, Alexey V Borisov, Natalya A Krivova, Yury V Kistenev

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Abstract

A quantitative assessment of wound status in a murine model was developed using phasor plot presentation of fluorescence lifetime imaging microscopy (FLIM) data. The quantitative assessment is based on calculating Bhattacharyya distance between g coordinates of FLIM data phasor plot density distributions of wound and healthy skin. The approach was validated for both diabetic and non-diabetic mice wounds, including during low-dose photodynamic therapy (LDPDT). Analysis revealed a shift in the FLIM data phasor plot g coordinates, suggesting altered metabolic processes involved in wound healing. Bhattacharyya distances in the LDPDT groups were closer to zero compared to the control group, which was not treated by LDPDT. Bhattacharyya distances in the non-diabetic LDPDT groups were closer to zero compared to the diabetic LDPDT groups that is consistent with the literature regarding the positive role of LDPDT in accelerating wound healing and the role of diabetes mellitus in impairing wound healing.

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利用 Phasor-FLIM 方法对糖尿病和非糖尿病皮肤伤口愈合进行体内定量评估

利用荧光寿命成像显微镜（FLIM）数据的相量图显示，开发了一种对小鼠模型伤口状态的定量评估方法。定量评估基于计算伤口和健康皮肤的 FLIM 数据相量图密度分布的 g 坐标之间的巴塔查里亚距离。该方法对糖尿病和非糖尿病小鼠伤口进行了验证，包括在低剂量光动力疗法（LDPDT）期间。分析表明，FLIM 数据相量图 g 坐标发生了变化，表明伤口愈合过程中的新陈代谢过程发生了改变。与未接受 LDPDT 治疗的对照组相比，LDPDT 组的巴塔克里亚距离更接近零。与糖尿病 LDPDT 组相比，非糖尿病 LDPDT 组的巴塔克里亚距离更接近零，这与有关 LDPDT 在加速伤口愈合方面的积极作用以及糖尿病在损害伤口愈合方面的作用的文献一致。

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Journal of biophotonics

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