Antimicrobial and cytotoxic effects of Lippia alba essential oil on multidrug-resistant Acinetobacter baumannii clinical isolates

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-10-30 DOI:10.1016/j.sajb.2024.10.019

Caroline Ferreira de Santana , Isis Caroline da Silva Cirino , Larissa Isabela Oliveira de Souza , Marcia Vanusa da Silva , Daniela Maria do Amaral Ferraz Navarro , Júlio César Ribeiro de Oliveira Farias de Aguiar , Regina Celia Bressan de Queiroz Figueiredo , Ana Raquel Pereira da Silva , Clara Mariana Gonçalves Lima , Evandro G. da Silva Jr , Henrique Douglas Melo Coutinho , Tereza Cristina Leal-Balbino

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Abstract

This study investigated the antimicrobial and cytotoxic effects of Lippia alba essential oil (LaEO) against five clinical isolates of multidrug-resistant A. baumannii from various infection sites in public hospitals in Recife, Pernambuco, Brazil. Gas chromatography-mass spectrometry analysis revealed 36 compounds in LaEO, with carvacrol (48.62 %), ρ-cymene (7.17 %), γ-terpinene (5.09 %), and thymol (5.00 %) as the most abundant constituents. LaEO exhibited potent inhibition of bacterial growth at MIC values ranging from 64 to 128 μg/mL, with MBC values similar to MIC. Additionally, interactions between LaEO and clinical antimicrobials (imipenem and gentamicin) demonstrated additive and synergistic effects when combined. Cytotoxicity assessment using the MTT assay on peritoneal macrophages revealed no significant difference in cell viability compared to the control, with a CC50 exceeding 512 μg/mL. Hemolytic activity on human red blood cells was minimal, with less than 2 % hemolysis observed at bacterially inhibitory concentrations.

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白芍药精油对耐多药鲍曼不动杆菌临床分离株的抗菌和细胞毒性作用

本研究调查了白芍药精油（LaEO）对来自巴西伯南布哥州累西腓市公立医院不同感染部位的五种耐多药鲍曼尼氏菌临床分离物的抗菌和细胞毒性作用。气相色谱-质谱分析显示，LaEO 中含有 36 种化合物，其中最丰富的成分是香芹酚（48.62%）、ρ-菊烯（7.17%）、γ-萜品烯（5.09%）和百里酚（5.00%）。LaEO 对细菌的生长有很强的抑制作用，其 MIC 值从 64 到 128 μg/mL 不等，MBC 值与 MIC 值相似。此外，LaEO 与临床抗菌药物（亚胺培南和庆大霉素）结合使用时，可产生相加和协同效应。利用 MTT 法对腹腔巨噬细胞进行的细胞毒性评估显示，与对照组相比，细胞存活率没有明显差异，CC50 超过 512 μg/mL。对人体红细胞的溶血活性极低，在细菌抑制浓度下观察到的溶血率低于 2%。

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