Transcriptional and Metabolomic Analyses Reveal That GmESR1 Increases Soybean Seed Protein Content Through the Phenylpropanoid Biosynthesis Pathway.

IF 6 1区 生物学 Q1 PLANT SCIENCES Plant, Cell & Environment Pub Date : 2024-11-01 DOI:10.1111/pce.15250
Runnan Zhou, Sihui Wang, Jianwei Li, Mingliang Yang, Chunyan Liu, Zhaoming Qi, Chang Xu, Xiaoxia Wu, Qingshan Chen, Ying Zhao
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Abstract

Soybeans are an economically vital food crop, which is employed as a key source of oil and plant protein globally. This study identified an EREBP-type transcription factor, GmESR1 (Enhance of Shot Regeneration). GmESR1 overexpression has been observed to significantly increase seed protein content. Furthermore, the molecular mechanism by which GmESR1 affects protein accumulation through transcriptome and metabolomics was also identified. The transcriptomic and metabolomic analyses identified 95 differentially expressed genes and 83 differentially abundant metabolites during the seed mid-maturity stage. Co-analysis strategies revealed that GmESR1 overexpression inhibited the biosynthesis of lignin, cellulose, hemicellulose, and pectin via the phenylpropane biosynthetic pathway, thereby redistributing biomass within cells. The key genes and metabolites impacted by this biochemical process included Gm4CL-like, GmCCR, Syringin, and Coniferin. Moreover, it was also found that GmESR1 binds to (AATATTATCATTAAGTACGGAC) during seed development and inhibits the transcription of GmCCR. GmESR1 overexpression also enhanced sucrose transporter gene expression during seed development and increased the sucrose transport rate. These results offer new insight into the molecular mechanisms whereby GmESR1 increases protein levels within soybean seeds, guiding future molecular-assisted breeding efforts aimed at establishing high-protein soybean varieties.

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转录和代谢组分析表明,GmESR1 可通过苯丙类生物合成途径增加大豆籽粒蛋白质含量。
大豆是一种具有重要经济价值的粮食作物,是全球油脂和植物蛋白的主要来源。这项研究发现了一种EREBP型转录因子--GmESR1(Enhance of Shot Regeneration)。据观察,过表达 GmESR1 可显著提高种子蛋白质含量。此外,还通过转录组学和代谢组学确定了 GmESR1 影响蛋白质积累的分子机制。转录组和代谢组分析确定了种子成熟中期的 95 个差异表达基因和 83 个差异丰度代谢物。协同分析策略显示,GmESR1 的过表达抑制了木质素、纤维素、半纤维素和果胶通过苯丙烷生物合成途径的生物合成,从而重新分配了细胞内的生物量。受这一生化过程影响的关键基因和代谢物包括 Gm4CL-like、GmCCR、Syringin 和 Coniferin。此外,研究还发现,在种子发育过程中,GmESR1 与(AATATTATCATTAAGTACGGAC)结合,抑制了 GmCCR 的转录。GmESR1 的过表达也增强了种子发育过程中蔗糖转运体基因的表达,并提高了蔗糖的转运率。这些结果为了解 GmESR1 提高大豆种子蛋白质水平的分子机制提供了新的视角,为今后旨在培育高蛋白大豆品种的分子辅助育种工作提供了指导。
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来源期刊
Plant, Cell & Environment
Plant, Cell & Environment 生物-植物科学
CiteScore
13.30
自引率
4.10%
发文量
253
审稿时长
1.8 months
期刊介绍: Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.
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