Construction of a hydrophilic porphyrin-based MOF@COF hybrid via post-synthetic modification for N-glycopeptides analysis in human serum.

IF 2.7 3区 化学 Q2 CHEMISTRY, ANALYTICAL Analytical Methods Pub Date : 2024-11-01 DOI:10.1039/d4ay01791a
Dongping Rao, Luyan Meng, Xiuqin Sheng, Wenying Yu, Chuan-Fan Ding, Yinghua Yan
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Abstract

A hybrid material, M@C-Br@GMA@Glu, consisting of a porphyrin-based metal-organic framework-covalent organic framework (MOF@COF), has been meticulously synthesized using a post-synthetic modification approach. This advanced material has demonstrated exceptional effectiveness in glycopeptide enrichment, characterized by an impressively low detection limit (0.2 fmol μL-1), high selectivity (1 : 2000), and loading capacity for glycopeptides (100 mg g-1). In practical applications with complex biological samples, 210 glycopeptides associated with 87 glycoproteins from the serum of the healthy group, and 156 glycopeptides related to 85 glycoproteins from the serum of cervical cancer were identified after enrichment with M@C-Br@GMA@Glu. Subsequent genetic ontology analysis has elucidated the relationship between cervical cancer and glycosylation, focusing on biological processes such as complement activation, innate immune response mechanisms, and the structural dynamics of the extracellular matrix. The collective findings not only validate the material's proficiency in the sensitive and selective enrichment of glycopeptides, but also underscore its potential in biomarker discovery applications.

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通过后合成修饰构建亲水性卟啉基 MOF@COF 混合物,用于分析人血清中的 N-糖肽。
我们采用后合成修饰方法精心合成了一种混合材料 M@C-Br@GMA@Glu,它由基于卟啉的金属有机框架-共价有机框架(MOF@COF)组成。这种先进的材料在富集糖肽方面表现出卓越的功效,其特点是检测限低(0.2 fmol μL-1)、选择性高(1:2000)、糖肽载量大(100 mg g-1)。在复杂生物样本的实际应用中,经 M@C-Br@GMA@Glu 富集后,从健康组血清中鉴定出与 87 种糖蛋白相关的 210 种糖肽,从宫颈癌血清中鉴定出与 85 种糖蛋白相关的 156 种糖肽。随后的基因本体分析阐明了宫颈癌与糖基化之间的关系,重点关注补体激活、先天性免疫反应机制和细胞外基质结构动态等生物过程。这些综合研究结果不仅验证了该材料在灵敏、选择性地富集糖肽方面的能力,还凸显了它在生物标记物发现应用方面的潜力。
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来源期刊
Analytical Methods
Analytical Methods CHEMISTRY, ANALYTICAL-FOOD SCIENCE & TECHNOLOGY
CiteScore
5.10
自引率
3.20%
发文量
569
审稿时长
1.8 months
期刊介绍: Early applied demonstrations of new analytical methods with clear societal impact
期刊最新文献
A sensitive SERS-based assay technique for accurate detection of foodborne pathogens without interference. High-throughput optimisations for 3D chemical imaging of pharmaceutical solid oral dosage forms. Rapid detection of Hg2+ in an ON-OFF-ON process using N doped carbon dots. Construction of a hydrophilic porphyrin-based MOF@COF hybrid via post-synthetic modification for N-glycopeptides analysis in human serum. Digoxin detection for therapeutic drug monitoring using target-triggered aptamer hairpin switch and nicking enzyme-assisted signal amplification.
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