The number and distribution of proliferating cells in the rat's rostral migratory stream as identified by means of two different proliferation markers.

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnic & Histochemistry Pub Date : 2024-11-01 DOI:10.1080/10520295.2024.2424252
Kamila Fabianová, Adam Raček, Alexandra Popovičová, Marcela Martončíková, Enikő Račeková
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Abstract

In the brains of adult rodents, the cells arising in the subventricular zone of the lateral ventricles maintain the ability to divide when migrating to the olfactory bulb along the rostral migratory stream (RMS). Dividing cells in the RMS are most frequently revealed through immunohistochemical detection of an exogenous marker of proliferation, 5-Bromo-2-deoxyuridine (BrdU), which incorporates into DNA during the S-phase of mitosis. The more recently recognized antigen Ki-67 (also known as Kiel-67 and MKI67), an endogenous protein expressed in nuclei at all stages of mitosis, is also used for proliferation detection. BrdU and Ki-67 are often used as alternative methods, but they have not previously been compared in the RMS. We analyzed the numbers and distribution of cells labeled either with BrdU or Ki-67 within the RMS of adult rats. The first group of animals received a single i.p. dose of BrdU. In the second group, dividing cells were visualized by Ki-67 immunohistochemistry. Some sections from brains of BrdU-treated rats were also immunostained for Ki-67. Labeled cells were counted in the three anatomical parts of the RMS (vertical arm, elbow and horizontal arm) using a method for unbiased estimation of cell density. The distribution of proliferating cells was similar for both markers. Most BrdU and Ki-67 positive cells were located in the vertical arm and in the elbow, but a caudo-rostral reduction in cell divisions was more evident with Ki-67 labeling. The number of Ki-67 positive cells significantly exceeded the number of BrdU positive cells in all parts of the RMS. Our results indicate that BrdU and Ki-67 are not interchangeable markers for evaluation of proliferative activity in the RMS.

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用两种不同的增殖标记物鉴定大鼠喙迁徙流中增殖细胞的数量和分布。
在成年啮齿动物的大脑中,产生于侧脑室室下区的细胞在沿着喙迁徙流(RMS)迁移到嗅球时保持分裂能力。RMS中的分裂细胞最常通过免疫组织化学方法检测外源性增殖标记物--5-溴-2-脱氧尿苷(BrdU)而被发现,该标记物在有丝分裂的S期结合到DNA中。最近被认可的抗原 Ki-67(又称 Kiel-67 和 MKI67)也可用于增殖检测,Ki-67 是有丝分裂各阶段细胞核中表达的内源性蛋白。BrdU和Ki-67经常被用作替代方法,但之前还没有在RMS中进行过比较。我们分析了成年大鼠 RMS 中用 BrdU 或 Ki-67 标记的细胞数量和分布情况。第一组动物接受了单剂量的BrdU静脉注射。第二组动物通过 Ki-67 免疫组织化学方法观察分裂细胞。经 BrdU 处理的大鼠大脑的部分切片也进行了 Ki-67 免疫染色。采用无偏估计细胞密度的方法,对RMS的三个解剖部位(垂直臂、肘部和水平臂)的标记细胞进行计数。两种标记的增殖细胞分布相似。大多数BrdU和Ki-67阳性细胞位于垂直臂和肘部,但Ki-67标记更明显地显示出细胞分裂的尾骶减少。在RMS的所有部位,Ki-67阳性细胞的数量都明显超过了BrdU阳性细胞的数量。我们的研究结果表明,BrdU和Ki-67并不是评估RMS增殖活性的可互换标记。
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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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