Comparative analysis of innate immune responses in Sonali and broiler chickens infected with tribasic H9N2 low pathogenic avian influenza virus.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES BMC Veterinary Research Pub Date : 2024-11-01 DOI:10.1186/s12917-024-04346-8
Ismail Hossain, Rupaida Akter Shila, Md Mohi Uddin, Emdadul Haque Chowdhury, Rokshana Parvin, Jahan Ara Begum
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Abstract

Background: H9N2 avian influenza viruses have been circulating in Bangladesh since 2006, affecting multiple avian species and resulting in economic losses. The recent emergence of tribasic strains, along with co-infections, has increased the risk to poultry health. Therefore, the study aimed to compare the immune responses of Sonali (crossbred) and commercial broiler chickens infected with tribasic H9N2 low pathogenic avian influenza (LPAI) virus.

Methods: Following H9N2 infection, proinflammatory (IL-6, IL-8, IL-1β and TNF-α) and antiviral (IFN-β and IFN-γ) cytokine expressions were observed in the trachea, lungs, intestine, and lymphoid tissues in Sonali and broiler chickens from 1 day post infection (dpi) to 10 dpi by qPCR.

Results: Sonali chickens exhibited significantly higher proinflammatory and antiviral cytokine expressions in the trachea at 3-7 days post infection (dpi), while broiler chickens showed lower immune responses. Broiler chickens displayed prolonged IL-6, IL-8, and IL-1β expression in lungs at 3-10 dpi compared to Sonali chickens. In the intestine, broiler chickens showed higher IL-6 and IL-8 expression that peaks at 1-3 dpi, while in Sonali chickens only IL-1β elevated at 10 dpi. In response to the H9N2 viruses, broiler chickens exhibited a stronger early IFN-β responses and a delayed IFN-γ responses in their lymphoid organs compared to Sonali chickens.

Conclusion: This suggests distinct immune profiles between the chicken types in response to the H9N2 infection. The information sheds light on the function of innate immunity in the pathophysiology of currently circulating tribasic H9N2 virus and could assist in effective controlling of avian influenza virus spread in poultry and designing vaccines.

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三型 H9N2 低致病性禽流感病毒感染索纳里鸡和肉鸡先天性免疫反应的比较分析。
背景:自 2006 年以来,H9N2 禽流感病毒一直在孟加拉国流行,影响多个禽类物种并造成经济损失。最近出现的三联株以及合并感染增加了家禽健康的风险。因此,本研究旨在比较感染三型 H9N2 低致病性禽流感(LPAI)病毒的 Sonali 鸡(杂交鸡)和商品肉鸡的免疫反应:感染 H9N2 病毒后,通过 qPCR 观察感染后 1 dpi 至 10 dpi 期间索娜丽鸡和肉鸡气管、肺、肠和淋巴组织中促炎细胞因子(IL-6、IL-8、IL-1β 和 TNF-α)和抗病毒细胞因子(IFN-β 和 IFN-γ)的表达:结果:感染后 3-7 天(dpi),索纳里鸡气管中的促炎细胞因子和抗病毒细胞因子表达量明显增加,而肉鸡的免疫反应较低。与索纳里鸡相比,肉鸡在感染后 3-10 dpi 肺部的 IL-6、IL-8 和 IL-1β 表达延长。在肠道中,肉鸡的 IL-6 和 IL-8 表达较高,在 1-3 dpi 达到峰值,而在 Sonali 鸡中,只有 IL-1β 在 10 dpi 出现升高。与索纳里鸡相比,肉鸡对 H9N2 病毒的早期 IFN-β 反应更强,而淋巴器官中的 IFN-γ 反应延迟:结论:这表明不同类型的鸡对 H9N2 感染有不同的免疫反应。这些信息揭示了先天免疫在目前流行的三型 H9N2 病毒病理生理学中的功能,有助于有效控制禽流感病毒在家禽中的传播和设计疫苗。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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Comparative analysis of innate immune responses in Sonali and broiler chickens infected with tribasic H9N2 low pathogenic avian influenza virus. Genetic characteristics and antimicrobial resistance of Staphylococcus aureus isolates from pig farms in Korea: emergence of cfr-positive CC398 lineage. Plasma N-terminal pro-B-type natriuretic peptide and urinary aldosterone-to-creatinine ratio in healthy Chihuahuas. Upper respiratory tract detection of Mycoplasma ovipneumoniae employing nasopharyngeal swabs. The clinical efficacy of cGMP-specific sildenafil on mitochondrial biogenesis induction and renal damage in cats with acute on chronic kidney disease.
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