Analytical methods for protein kinase and inhibitor screening including kinetic evaluation

IF 11.8 1区 化学 Q1 CHEMISTRY, ANALYTICAL Trends in Analytical Chemistry Pub Date : 2024-10-26 DOI:10.1016/j.trac.2024.118025
Viola Wurster , Peter Fechner , Günther Proll , Carolin Huhn
{"title":"Analytical methods for protein kinase and inhibitor screening including kinetic evaluation","authors":"Viola Wurster ,&nbsp;Peter Fechner ,&nbsp;Günther Proll ,&nbsp;Carolin Huhn","doi":"10.1016/j.trac.2024.118025","DOIUrl":null,"url":null,"abstract":"<div><div>Protein kinases promote cell processes such as cell growth and proliferation similarly in tumor cells and healthy cells. Pharmaceutical research aims at developing small molecules that inhibit tumor growth binding in the ATP-binding pocket of specific protein kinases. To understand the specificity of the inhibitor, thermodynamic and kinetic data of the kinase-inhibitor binding process are important. A broad range of analytical methods may investigate the interactions between protein kinases and inhibitors. Today, however, there is no comprehensive strategy to provide information on both specificity screening and binding kinetics. The purpose of this review is to summarize analytical methods used not only to monitor the protein kinase inhibition, but also to provide kinetic and thermodynamic data on the basis of screening applications. A focus is set on optical biosensing as the most promising techniques. We discuss, that intelligent combinations of methods can provide comprehensive information on protein kinases interacting with inhibitors.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"181 ","pages":"Article 118025"},"PeriodicalIF":11.8000,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in Analytical Chemistry","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165993624005089","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Protein kinases promote cell processes such as cell growth and proliferation similarly in tumor cells and healthy cells. Pharmaceutical research aims at developing small molecules that inhibit tumor growth binding in the ATP-binding pocket of specific protein kinases. To understand the specificity of the inhibitor, thermodynamic and kinetic data of the kinase-inhibitor binding process are important. A broad range of analytical methods may investigate the interactions between protein kinases and inhibitors. Today, however, there is no comprehensive strategy to provide information on both specificity screening and binding kinetics. The purpose of this review is to summarize analytical methods used not only to monitor the protein kinase inhibition, but also to provide kinetic and thermodynamic data on the basis of screening applications. A focus is set on optical biosensing as the most promising techniques. We discuss, that intelligent combinations of methods can provide comprehensive information on protein kinases interacting with inhibitors.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
筛选蛋白激酶和抑制剂的分析方法,包括动力学评估
蛋白激酶促进细胞生长和增殖等细胞过程,肿瘤细胞和健康细胞的情况类似。药物研究旨在开发能抑制肿瘤生长的小分子药物,这些药物能与特定蛋白激酶的 ATP 结合袋结合。要了解抑制剂的特异性,激酶-抑制剂结合过程的热力学和动力学数据非常重要。有多种分析方法可以研究蛋白激酶与抑制剂之间的相互作用。然而,目前还没有一种全面的策略能同时提供特异性筛选和结合动力学方面的信息。本综述旨在总结不仅用于监测蛋白激酶抑制作用,而且用于在筛选应用的基础上提供动力学和热力学数据的分析方法。重点放在最有前途的光学生物传感技术上。我们讨论了各种方法的智能组合可以提供蛋白激酶与抑制剂相互作用的全面信息。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Trends in Analytical Chemistry
Trends in Analytical Chemistry 化学-分析化学
CiteScore
20.00
自引率
4.60%
发文量
257
审稿时长
3.4 months
期刊介绍: TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.
期刊最新文献
Determination and assessment of contamination of toxic chemical elements in soils – Review Recent advances in X-ray grating-based dark-field imaging Integrated breath analysis technologies: Current advances and future prospects Advancements in CRISPR-diagnostic techniques for rapid on-site monitoring of environmental virus Evaluating excitation-emission matrix for characterization of dissolved organic matter in natural and engineered water systems: Unlocking submerged secrets
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1