Ethyl methanesulfonate (EMS) mediated dwarfing mutation provides a basis for CaCO3 accumulation by enhancing photosynthetic performance in Ceratostigma willmottianum Stapf

IF 4.2 3区 生物学 Q1 PLANT SCIENCES Plant Biology Pub Date : 2024-11-05 DOI:10.1111/plb.13737
L. Guo, J. Lai, T. Lei, C. Liu, J. Li, L. Yang, S. Gao
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甲磺酸乙酯(EMS)介导的矮化突变通过提高 Ceratostigma willmottianum Stapf.
Ceratostigma willmottianum Stapf 是中国特有的白垩腺(盐分泌)植物,其盐腺结构可排出白色碳酸钙(CaCO3)晶体,具有潜在的生物矿化和固碳功能。由于野生种质资源分布狭窄,新品种缺乏多样性,难以满足商业开发和科学探索的需要。因此,我们利用甲基磺酸乙酯(EMS)诱变技术获得了新的矮化突变种质,并对其形态、生长、光合作用、盐腺和排泄物等性状进行了分析。四个矮化突变株系(DM1、DM2、DM3 和 DM4)均表现出极度矮化(株高分别降低 62.28%、62.28%、74.55% 和 61.68%)、生长速度加快、地下根生物量增加、花芽分化和开花提前。光合能力增强:DM 突变体叶片的叶绿素含量、PSII 最大量子产率(Fv/Fm)、PSII 有效量子产率(ΦPSII)、光化学淬灭系数(qP)、电子转移率(ETR)、净光合作用(Pn)、细胞间 CO2 浓度(Ci)、气孔导度(Gs)和蒸腾作用(Tr)均显著提高。单位叶面积的盐腺密度和单个盐腺的平均 Ca2+ 排泄速率明显增加(尤其是 DM2),单位叶面积的 CaCO3 积累比野生型高 28.57%。皮尔逊相关分析表明,光合作用能力与 CaCO3 的排泄量呈显著正相关。上述研究不仅丰富了C. willmottianum的新种质,而且为研究盐腺排泄CaCO3的机理和生物矿化固碳能力提供了重要的研究材料。
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来源期刊
Plant Biology
Plant Biology 生物-植物科学
CiteScore
8.20
自引率
2.60%
发文量
109
审稿时长
3 months
期刊介绍: Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology. Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.
期刊最新文献
Issue Information Variation in suppression of black-grass by modern and ancestral cereal root exudates. Monkey as seed dispersers of Neotropical Vanilla involves social learning. Different tools for different trades: contrasts in specialized metabolite chemodiversity and phylogenetic dispersion in fruit, leaves, and roots of the neotropical shrubs Psychotria and Palicourea (Rubiaceae). Chemical, morphological, and genetic characterization of the floral scent and scent-releasing structures of Gynandropsis gynandra (Cleomaceae, Brassicales).
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