The first intron and promoter of Arabidopsis DIACYLGLYCEROL ACYLTRANSFERASE 1 exert synergistic effects on pollen and embryo lipid accumulation

IF 8.1 1区 生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2024-11-05 DOI:10.1111/nph.20244
Sean T. McGuire, Jay Shockey, Philip D. Bates
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拟南芥二乙酰甘油 ACYLTRANSFERASE 1 的第一个内含子和启动子对花粉和胚胎脂质积累有协同作用。
三酰甘油(TAG)的积累在植物发育的各个阶段都至关重要。在拟南芥中,有两种酶具有产生 TAGs 的重叠功能,即酰基-CoA:二酰甘油酰基转移酶 1(DGAT1)和磷脂:二酰甘油酰基转移酶 1(PDAT1)。dgat1-1/pdat1-2 双突变体中这两个基因的功能缺失会导致配子体致死。然而,控制这两个基因组织特异性表达的关键调控元件尚未确定。我们用含有拟南芥 DGAT1 启动子的转基因构建体转化了 dgat1-1/dgat1-1//PDAT1/pdat1-2 亲本,该构建体融合了带有或不带有第一个内含子的 AtDGAT1 开放阅读框。然而,在没有 DGAT1 第一个内含子的情况下,花药不能充满花粉,种子产量约为野生型的 10%,种子含油量仍然降低(与 dgat1-1/dgat1-1 相似),并且 PDAT1/pdat1-2 基因座出现非孟德尔分离。而表达含有第一个内含子的 AtDGAT1pro:AtDGAT1 转基因的植株,其表型大多恢复为野生型。这项研究证实,AtDGAT1启动子和第一个内含子的结合为AtDGAT1在花粉中的时间和组织特异性表达提供了适当的背景。此外,我们还讨论了内含子介导调控的可能机制,以及如何利用调控元件作为遗传工具,在拟南芥中实现 TAG 生物合成酶的功能替代。
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来源期刊
New Phytologist
New Phytologist 生物-植物科学
自引率
5.30%
发文量
728
期刊介绍: New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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