Microextraction by packed sorbent: Introducing a novel hybrid silica-based chitosan-graphene oxide biosorbent for the evaluation of pesticides and antibiotics in food matrices

IF 5.2 Q1 CHEMISTRY, ANALYTICAL Advances in Sample Preparation Pub Date : 2024-10-01 DOI:10.1016/j.sampre.2024.100134
Rafael O. Martins, Fernando M. Lanças
{"title":"Microextraction by packed sorbent: Introducing a novel hybrid silica-based chitosan-graphene oxide biosorbent for the evaluation of pesticides and antibiotics in food matrices","authors":"Rafael O. Martins,&nbsp;Fernando M. Lanças","doi":"10.1016/j.sampre.2024.100134","DOIUrl":null,"url":null,"abstract":"<div><div>This study introduces a novel silica-graphene oxide@chitosan (SiGO@CS) material as a packed biosorbent for microextraction by packed sorbent (MEPS), followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of pesticides (atrazine and thiamethoxam) and antibiotics (ceftiofur and sulfonamide) in food samples. The graphene-based aerogel was modified with varying percentages of silica-graphene oxide/chitosan (w/w) and characterized to confirm successful chitosan incorporation. Optimization of the MEPS protocol, using 2<sup>4–1</sup> and 2<sup>3</sup> experimental designs, identified draw/eject and washing cycles as the most influential parameters for extraction efficiency. The SiGO@CS biosorbent with 80 % CS/SiGO (w/w) exhibited superior extraction efficiency compared to other ratios and commercial sorbents. The method demonstrated excellent linearity for all analytes (R² &gt; 0.9900), with low limits of detection (LOD) and quantification (LOQ) ranging from 0.020 to 0.045 µg <span>l</span><sup>-1</sup> and 0.045 to 1.0 µg <span>l</span><sup>-1</sup> for pesticides, respectively, and 5 to 15 µg <span>l</span><sup>-1</sup> and 15 to 20 µg <span>l</span><sup>-1</sup> for antibiotics, respectively. Trueness values were within 82 % to 109 %. The method's green credentials were confirmed using AGREEprep and the Green Analytical Procedure Index (GAPI) approach, highlighting sorbent reusability (over 15 times) and rapid analytical throughput (5 min per sample) with low use of pre-treated sample extract volume (500 µL). The application to local corn, tomato, and milk samples confirmed the detection and quantification of thiamethoxam and atrazine at concentrations above the recommended ingestion per day for one sample of tomato and corn out of the three samples analyzed. Furthermore, using the novel SiGO@CS biosorbent in the MEPS protocol offers a green, high-performance analytical alternative to traditional sorbent phases, with the potential for evaluating trace levels of pesticides and antibiotics in food matrices.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"12 ","pages":"Article 100134"},"PeriodicalIF":5.2000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Sample Preparation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772582024000329","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

This study introduces a novel silica-graphene oxide@chitosan (SiGO@CS) material as a packed biosorbent for microextraction by packed sorbent (MEPS), followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis of pesticides (atrazine and thiamethoxam) and antibiotics (ceftiofur and sulfonamide) in food samples. The graphene-based aerogel was modified with varying percentages of silica-graphene oxide/chitosan (w/w) and characterized to confirm successful chitosan incorporation. Optimization of the MEPS protocol, using 24–1 and 23 experimental designs, identified draw/eject and washing cycles as the most influential parameters for extraction efficiency. The SiGO@CS biosorbent with 80 % CS/SiGO (w/w) exhibited superior extraction efficiency compared to other ratios and commercial sorbents. The method demonstrated excellent linearity for all analytes (R² > 0.9900), with low limits of detection (LOD) and quantification (LOQ) ranging from 0.020 to 0.045 µg l-1 and 0.045 to 1.0 µg l-1 for pesticides, respectively, and 5 to 15 µg l-1 and 15 to 20 µg l-1 for antibiotics, respectively. Trueness values were within 82 % to 109 %. The method's green credentials were confirmed using AGREEprep and the Green Analytical Procedure Index (GAPI) approach, highlighting sorbent reusability (over 15 times) and rapid analytical throughput (5 min per sample) with low use of pre-treated sample extract volume (500 µL). The application to local corn, tomato, and milk samples confirmed the detection and quantification of thiamethoxam and atrazine at concentrations above the recommended ingestion per day for one sample of tomato and corn out of the three samples analyzed. Furthermore, using the novel SiGO@CS biosorbent in the MEPS protocol offers a green, high-performance analytical alternative to traditional sorbent phases, with the potential for evaluating trace levels of pesticides and antibiotics in food matrices.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
利用填料吸附剂进行微萃取:采用新型壳聚糖-氧化石墨烯混合硅基生物吸附剂评估食品基质中的农药和抗生素
本研究介绍了一种新型二氧化硅-氧化石墨烯@壳聚糖(SiGO@CS)材料,它是一种填料生物吸附剂,可用于填料吸附剂微萃取(MEPS),然后通过液相色谱-串联质谱(LC-MS/MS)分析食品样品中的农药(阿特拉津和噻虫嗪)和抗生素(头孢噻呋和磺酰胺)。用不同比例的二氧化硅-氧化石墨烯/壳聚糖(重量比)对石墨烯基气凝胶进行了改性,并对其进行了表征,以确认壳聚糖的成功加入。采用 24-1 和 23 实验设计对 MEPS 方案进行了优化,确定抽吸/喷射和洗涤周期是对萃取效率影响最大的参数。与其他比例和商用吸附剂相比,SiGO@CS 生物吸附剂(CS/SiGO(重量比)为 80%)的萃取效率更高。该方法对所有分析物均表现出良好的线性关系(R² > 0.9900),检出限(LOD)和定量限(LOQ)较低,农药的检出限(LOD)和定量限(LOQ)分别为 0.020 至 0.045 µg l-1 和 0.045 至 1.0 µg l-1,抗生素的检出限(LOD)和定量限(LOQ)分别为 5 至 15 µg l-1 和 15 至 20 µg l-1。真实值在 82 % 至 109 % 之间。使用 AGREEprep 和绿色分析程序指数 (GAPI) 方法确认了该方法的绿色证书,突出了吸附剂的可重复使用性(超过 15 次)和快速分析吞吐量(每个样品 5 分钟),且预处理样品提取物用量少(500 µL)。对当地玉米、番茄和牛奶样品的应用证实,在分析的三个样品中,有一个番茄和一个玉米样品的噻虫嗪和阿特拉津的检测和定量浓度超过了建议的每日摄入量。此外,在 MEPS 方案中使用新型 SiGO@CS 生物吸附剂为传统吸附剂相提供了一种绿色、高性能的分析替代品,有望评估食品基质中痕量农药和抗生素的含量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
3.50
自引率
0.00%
发文量
0
期刊最新文献
Determination of steroid hormones in sea urchins by microwave-assisted extraction and ultrahigh-performance liquid chromatography tandem mass spectrometry Extraction of synthetic cathinones from biological samples: A systematic review Microextraction by packed sorbent: Introducing a novel hybrid silica-based chitosan-graphene oxide biosorbent for the evaluation of pesticides and antibiotics in food matrices Simultaneous and sequential combination of techniques for the sustainable and extensive extraction of proteins and polyphenols from malt rootlets Improving sample preparation by biochar-coated sampling tubes: proof-of-concept extraction of sex hormones from real waters
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1