Seeing tension in cells

IF 17.3 1区 生物学 Q1 CELL BIOLOGY Nature Cell Biology Pub Date : 2024-11-11 DOI:10.1038/s41556-024-01562-0
Daryl J. V. David
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看到细胞中的张力
非肌肉肌球蛋白 II(NMII)会产生收缩力,而基于佛斯特共振能量转移(FRET)的张力传感器可以测量细胞中的张力。哈特等人开发了一种基于 FRET 的机械生物传感器,它以 NMII 为基础,适用于活体细胞成像。作者改造了一种张力传感器模块,该模块的 FRET 效率在暴露于机械拉伸造成的物理伸展后会降低,并将其设计到 NMII 中。作者在多种细胞类型中对其进行了测试,利用光漂白后的荧光恢复,发现张力传感器 NMII 构建物和荧光标记 NMII 的周转率相似。他们发现,张力传感器融入了内源性 NMII 细丝,而且其构建物的 FRET 效率对药理肌球蛋白扰动很敏感。
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来源期刊
Nature Cell Biology
Nature Cell Biology 生物-细胞生物学
CiteScore
28.40
自引率
0.90%
发文量
219
审稿时长
3 months
期刊介绍: Nature Cell Biology, a prestigious journal, upholds a commitment to publishing papers of the highest quality across all areas of cell biology, with a particular focus on elucidating mechanisms underlying fundamental cell biological processes. The journal's broad scope encompasses various areas of interest, including but not limited to: -Autophagy -Cancer biology -Cell adhesion and migration -Cell cycle and growth -Cell death -Chromatin and epigenetics -Cytoskeletal dynamics -Developmental biology -DNA replication and repair -Mechanisms of human disease -Mechanobiology -Membrane traffic and dynamics -Metabolism -Nuclear organization and dynamics -Organelle biology -Proteolysis and quality control -RNA biology -Signal transduction -Stem cell biology
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