β-Hydroxy β-Methyl Butyric Acid (HMB) and Its Potential Doping Relevance: A Pilot Study on Its Urinary Excretion Profile.

IF 2.6 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS Drug Testing and Analysis Pub Date : 2024-11-07 DOI:10.1002/dta.3826

Cristian Camuto, Xavier de la Torre, Francesco Botrè, Fabio de Giorgio

引用次数: 0

Abstract

β-hydroxy β-methyl butyric acid (HMB), either as the free acid or in the form of its calcium salt, is a component of several dietary supplements marketed to enhance sports performance, due to its role in protein synthesis. In this pilot study, we investigated the urinary excretion profile of HMB, an endogenous metabolite of the essential amino acid leucine. The endogenous reference ranges of HMB, in human urine samples, were monitored by collecting samples from 20 volunteers. Data obtained were compared with those from controlled excretion studies, following the intake of a 3-g oral dose of HMB. Urine samples were analyzed through a targeted procedure employing a conventional GC-MS system operating in SIM mode. Our results show that the excreted urinary concentration of HMB significantly exceeds the range of endogenous concentrations for at least 24 h, making possible to detect its exogenous origin.

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β-羟基 β-甲基丁酸 (HMB) 及其潜在的兴奋剂相关性：关于其尿液排泄特征的试点研究。

β-羟基β-甲基丁酸（HMB）是一种游离酸或其钙盐形式的物质，由于其在蛋白质合成中的作用，因此是市场上销售的几种用于提高运动成绩的膳食补充剂的成分之一。在这项试验研究中，我们调查了人体必需氨基酸亮氨酸的内源性代谢产物 HMB 的尿液排泄情况。通过采集 20 名志愿者的尿样，对人体尿样中 HMB 的内源性参考范围进行了监测。在口服 3 克剂量的 HMB 后，将获得的数据与对照排泄研究的数据进行比较。尿液样本通过采用传统 GC-MS 系统在 SIM 模式下运行的定向程序进行分析。结果表明，至少在 24 小时内，尿液中排出的 HMB 浓度明显超过了内源性浓度的范围，因此可以检测出其外源性来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

5.90

自引率

24.10%

发文量

191

审稿时长

2.3 months

期刊介绍： As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.