Photoinduced Electrochemiluminescence Immunoassays

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL Analytical Chemistry Pub Date : 2024-11-01 DOI:10.1021/acs.analchem.4c0466210.1021/acs.analchem.4c04662

Dongni Han, Jasmina Vidic, Dechen Jiang*, Gabriel Loget* and Neso Sojic*,

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Abstract

Optimization of electrochemiluminescence (ECL) immunoassays is highly beneficial for enhancing clinical diagnostics. A major challenge is the improvement of the operation conditions required for the bead-based immunoassays using the typical [Ru(bpy)₃]²⁺/tri-n-propylamine (TPrA) system. In this study, we report a heterogeneous immunoassay based on near-infrared photoinduced ECL, which facilitates the imaging and quantitative analysis of [Ru(bpy)₃]²⁺-modified immunobeads at low anodic potential. The photovoltage generated by the photoanode under near-infrared light promotes oxidation processes at the electrode/electrolyte interface, thus considerably lowering the onset potential for both TPrA oxidation and ECL emission. The anti-Stokes shift between the excitation light (invisible to the human eyes) and the visible emitted light results in a clear and stable signal from the immunobeads. In addition, it offers the possibility of site-selective photoexcitation of the ECL process. This approach not only meets the performance of traditional ECL immunoassays in accuracy but also offers the additional benefits of lower potential requirements and enhanced stability, providing a new perspective for the optimization of commercial immunoassays.

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光诱导电化学发光免疫分析仪

电化学发光（ECL）免疫测定的优化非常有利于提高临床诊断水平。一个主要的挑战是如何改善使用典型的[Ru(py)3]2+/三正丙胺（TPrA）体系的珠基免疫分析所需的操作条件。在本研究中，我们报告了一种基于近红外光诱导 ECL 的异质免疫分析法，它有助于在低阳极电位下对[Ru(镱)3]2+ 修饰的免疫eads 进行成像和定量分析。光阳极在近红外光下产生的光电压促进了电极/电解质界面的氧化过程，从而大大降低了 TPrA 氧化和 ECL 发射的起始电位。激发光（人眼不可见）和可见发射光之间的反斯托克斯偏移导致免疫eads 发出清晰稳定的信号。此外，它还为 ECL 过程的位点选择性光激发提供了可能。这种方法不仅在准确性上达到了传统 ECL 免疫测定的性能要求，而且还具有降低电位要求和提高稳定性的额外优势，为商业免疫测定的优化提供了新的视角。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.