Knockdown of DDX3Y alleviates ovalbumin-induced allergic rhinitis in mice by regulating NF-κB pathway.

IF 2.5 4区医学 Q3 ALLERGY Allergologia et immunopathologia Pub Date : 2024-11-01 eCollection Date: 2024-01-01 DOI:10.15586/aei.v52i6.1156

Ying Xu, Xiaofeng Gu, Jian Wu, Wenmin Lu

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Abstract

Allergic rhinitis (AR), a type of chronic inflammatory disease that exists in the nasal mucosa, significantly impacts the quality of life. DDX3Y gene encodes an RNA helicase belonging to the DEAD-box protein family and is part of the DDX3 subfamily that affects the progression of multiple diseases. However, the specific role and mechanisms of DDX3Y in AR remain unclear. This study investigates the effects of DDX3Y knockdown on ovalbumin (OVA)-induced AR in mice. We found that DDX3Y is highly expressed in the nasal mucosa of AR mice. Knockdown of DDX3Y in OVA-induced AR mice significantly alleviated nasal manifestations, reduced immunoglobulin E and histamine levels, and improved nasal mucosal histopathology. Additionally, knockdown of DDX3Y suppressed secretion of inflammatory factor nuclear factor kappa B (NF-κB) phosphorylation, thereby mitigating local inflammatory responses. These findings suggested that targeting DDX3Y could offer a novel therapeutic strategy for managing AR by modulating the NF-κB pathway.

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通过调节 NF-κB 通路，敲除 DDX3Y 可减轻卵清蛋白诱发的小鼠过敏性鼻炎。

过敏性鼻炎（AR）是一种存在于鼻粘膜的慢性炎症性疾病，严重影响患者的生活质量。DDX3Y 基因编码一种属于 DEAD-box 蛋白家族的 RNA 螺旋酶，是影响多种疾病进展的 DDX3 亚家族的一部分。然而，DDX3Y在AR中的具体作用和机制仍不清楚。本研究探讨了敲除 DDX3Y 对卵清蛋白（OVA）诱导的小鼠 AR 的影响。我们发现 DDX3Y 在 AR 小鼠的鼻粘膜中高表达。在 OVA 诱导的 AR 小鼠中敲除 DDX3Y 能显著缓解鼻部表现，降低免疫球蛋白 E 和组胺水平，并改善鼻粘膜组织病理学。此外，DDX3Y的敲除抑制了炎症因子核因子卡巴B（NF-κB）磷酸化的分泌，从而减轻了局部炎症反应。这些研究结果表明，以DDX3Y为靶点可以通过调节NF-κB通路为控制AR提供一种新的治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Allergologia et immunopathologia 医学-过敏

CiteScore

3.70

自引率

0.00%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.