Incipient resistance of the African maize stemborer, Busseola fusca (Lepidoptera: Noctuidae) to Cry2Ab2 maize in South Africa.

IF 3.8 1区农林科学 Q1 AGRONOMY Pest Management Science Pub Date : 2024-11-14 DOI:10.1002/ps.8529

Elrine Strydom, Annemie Erasmus, Stephanus Friis, Jacques Magson, Srinivas Parimi, Samuel Martinelli, Graham P Head, Hannalene du Plessis, Johnnie van den Berg

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Abstract

Background: Resistance of Busseola fusca (Lepidoptera: Noctuidae) to Cry1Ab was documented in 2006, 7 years after the first cultivation of MON 810 in South Africa. This was mitigated by introducing a second-generation Bacillus thuringiensis (Bt) maize (MON 89034), which contains the Cry1A.105 and Cry2Ab2 proteins. The first reports of B. fusca infestations of MON 89034 maize came in the KwaZulu-Natal province (2017-2018 cropping season), followed by reports in the Mpumalanga province (2022-2023 season). Here we report results of artificial diet- and plant-based laboratory assays to assess the susceptibility of B. fusca populations to the Bt proteins in MON 89034.

Results: Larvae were sampled from nine locations which included three where greater than expected injury due to B. fusca had been reported to MON 89034. Larval mortality in assays with diet-incorporated Cry2Ab2 protein was 100% for all except the three problem populations, showing that the Cry2Ab2 protein in MON 89034 is highly efficacious against B. fusca. In contrast, assays with Cry1A.105 did not cause significant mortality in any of the B. fusca populations including larvae from a susceptible reference. Larval survival on leaf tissue of MON 89034 maize after 7 days ranged between 75% and 91% for the three problematic B. fusca populations, compared to 0.4% to 9.6% for the five other populations.

Conclusion: Therefore, MON 89034 is effectively a single-mode-of-action technology against B. fusca and carries an inherent high risk for the evolution of resistance. This study shows that the three B. fusca populations collected from locations with greater than expected damage to MON 89034 have resistance to the Cry2Ab2 protein and therefore to MON 89034 maize. This research emphasizes the importance of resistance monitoring and implementation of effective insect resistance management tactics. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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南非非洲玉米螟 Busseola fusca（鳞翅目：夜蛾科）对 Cry2Ab2 玉米的初期抗性。

背景：2006 年，即在南非首次种植 MON 810 玉米 7 年后，发现 Busseola fusca（鳞翅目：夜蛾科）对 Cry1Ab 产生抗性。通过引入含有 Cry1A.105 和 Cry2Ab2 蛋白质的第二代苏云金芽孢杆菌（Bt）玉米（MON 89034），这一问题得到了缓解。首批关于MON 89034玉米遭受镰刀菌侵染的报告出现在夸祖鲁-纳塔尔省（2017-2018种植季），随后在姆普马兰加省（2022-2023种植季）也有报告。在此，我们报告了基于人工饮食和植物的实验室检测结果，以评估蚕蛾种群对 MON 89034 中 Bt 蛋白的敏感性：结果：从九个地点对幼虫进行了采样，其中三个地点曾有报告称 MON 89034 对镰刀菌造成的伤害超过了预期。除三个有问题的种群外，在使用融入食物的 Cry2Ab2 蛋白的试验中，所有种群的幼虫死亡率均为 100%，这表明 MON 89034 中的 Cry2Ab2 蛋白对镰刀菌非常有效。与此相反，Cry1A.105 的检测对任何镰刀菌种群（包括来自易感参照物的幼虫）都没有造成明显的死亡。7 天后，在 MON 89034 玉米叶片组织上的幼虫存活率在 75% 到 91% 之间，而在三个有问题的镰刀菌种群上的幼虫存活率在 0.4% 到 9.6% 之间，在其他五个种群上的幼虫存活率在 0.4% 到 9.6% 之间：因此，MON 89034 实际上是一种针对镰刀菌的单一作用模式技术，具有抗性演变的内在高风险。这项研究表明，从对 MON 89034 产生超出预期危害的地点采集的三个镰刀菌种群对 Cry2Ab2 蛋白产生抗性，因此也对 MON 89034 玉米产生抗性。这项研究强调了抗性监测和实施有效的昆虫抗性管理策略的重要性。© 2024 作者。害虫管理科学》由 John Wiley & Sons Ltd 代表化学工业协会出版。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.