The Effect of Osteoprotegerin (OPG)/Receptor Activator of Nuclear Factor-κB Ligand (RANKL)/Receptor Activator of Nuclear Factor-κB (RANK) on Aortic Valve Calcified.

IF 1.1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY Annals of clinical and laboratory science Pub Date : 2024-09-01

Wei Luo, Jing Wang, Xia Yang, Junshan Li, Yanqiu Song, Hongliang Cong

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Abstract

Objective: To evaluate the effect of osteoprotegerin (OPG)/receptor activator of nuclear factor-[Formula: see text]B ligand (RANKL)/receptor activator of nuclear factor-[Formula: see text]B (RANK) nuclear factor on aortic valve calcification.

Methods: The aortic valve tissue was collected from 132 aortic stenosis (AS) patients who underwent valve replacement. The valve tissue was stained with hematoxylin eosin (HE) and alizarin red calcium salt deposition. At the same time, CD68, RUNX2, TRAP immunohistochemical staining and double staining were performed.

Results: ELISA analysis showed that the peripheral blood OPG value in the severe calcification group was significantly higher than mild calcification group (P=0.000) and non-calcification group (P=0.000), while the content of peripheral blood sRANKL in the severe calcification group was significantly lower than that in the non-calcification group (P=0.001). The valval OPG value in the mild calcification group was significantly higher than that of the non-calcification group (P=0.001) and the severe calcification group (P=0.040), and the valval sRANKL value of the severe calcification group was significantly lower than that of the non-calcification group (P=0.000).

Conclusion: The expression of OPG and RANKL can regulate the inflammatory reaction of valve and the balance between bone formation and resorption, thus affecting the progress of valve calcification.

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骨蛋白激酶（OPG）/核因子κB受体活化因子配体（RANKL）/核因子κB受体活化因子（RANK）对主动脉瓣钙化的影响。

目的评估骨保护素（OPG）/核因子受体活化因子-[式：见正文]B配体（RANKL）/核因子受体活化因子-[式：见正文]B（RANK）核因子对主动脉瓣钙化的影响：方法：收集了132名接受瓣膜置换术的主动脉瓣狭窄（AS）患者的主动脉瓣组织。瓣膜组织经苏木精-伊红（HE）和茜素红钙盐沉积染色。同时进行 CD68、RUNX2、TRAP 免疫组化染色和双重染色：ELISA分析显示，重度钙化组外周血OPG值明显高于轻度钙化组（P=0.000）和非钙化组（P=0.000），而重度钙化组外周血sRANKL含量明显低于非钙化组（P=0.001）。轻度钙化组的OPG值明显高于非钙化组（P=0.001）和重度钙化组（P=0.040），重度钙化组的sRANKL值明显低于非钙化组（P=0.000）：结论：OPG和RANKL的表达可调节瓣膜的炎症反应和骨形成与吸收之间的平衡，从而影响瓣膜钙化的进程。

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来源期刊

Annals of clinical and laboratory science 医学-医学实验技术

CiteScore

1.60

自引率

0.00%

发文量

112

审稿时长

6-12 weeks

期刊介绍： The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.