Campylobacter sputorum subsp. bovis subsp. nov., isolated from cattle, and an emended description of Campylobacter sputorum.

IF 2 3区 生物学 Q4 MICROBIOLOGY International journal of systematic and evolutionary microbiology Pub Date : 2024-11-01 DOI:10.1099/ijsem.0.006571
William G Miller, Tina G Williams, Delilah F Wood, Mary H Chapman
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Abstract

Six urease-negative Campylobacter strains were isolated from cattle faeces over a 19-month period from 2009 to 2010. These strains were initially identified as Campylobacter sputorum by 16S rRNA gene and atpA typing. Initial studies characterizing these strains by multilocus sequence typing and genome sequencing further supported their classification as C. sputorum but indicated that these strains form a divergent clade within the species. A polyphasic study was undertaken here to clarify their taxonomic position. Phylogenetic analyses were performed based on 16S rRNA gene sequences and the concatenated sequences of 330 core genes, with the latter analysis also placing the six strains into a clade distinct from the three C. sputorum biovars. Pairwise digital DNA-DNA hybridization values identified these strains as C. sputorum, and the pairwise average nucleotide identity values were consistent with those observed between current Campylobacter subspecies pairs. Standard phenotypic testing was also performed. All strains are microaerobic, anaerobic, motile, Gram-negative and oxidase- and catalase-positive; cells are curved rods or spirals. Strains can be distinguished from the C. sputorum biovars by the presence of alkaline phosphatase activity and triphenyltetrazolium chloride reduction and absence of nitrate reduction. The data presented here show that these strains represent a novel subspecies within C. sputorum, for which the name C. sputorum subsp. bovis subsp. nov. (type strain RM8705T=LMG 32300T=CCUG 75470T) is proposed.

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Campylobacter sputorum subsp.
在 2009 年至 2010 年的 19 个月期间,从牛粪便中分离出六株尿素酶阴性弯曲杆菌。通过 16S rRNA 基因和 atpA 分型,这些菌株被初步鉴定为唾液弯曲杆菌。通过多焦点序列分型和基因组测序对这些菌株进行特征描述的初步研究进一步支持将其归类为C. sputorum,但表明这些菌株在该物种中形成了一个分化支系。为了明确这些菌株在分类学上的位置,我们在此开展了一项多相研究。根据 16S rRNA 基因序列和 330 个核心基因的连接序列进行了系统发育分析,后一项分析也将这六株菌株归入一个支系,与三个 C. sputorum 生物变种截然不同。成对的数字 DNA-DNA 杂交值确定这些菌株为 C. sputorum,成对的平均核苷酸同一性值与目前弯曲杆菌亚种对之间的同一性值一致。此外,还进行了标准的表型检测。所有菌株均为微需氧、厌氧、运动、革兰氏阴性、氧化酶和过氧化氢酶阳性;细胞为弯曲棒状或螺旋状。通过碱性磷酸酶活性和三苯基氯化四氮唑还原作用的存在以及硝酸盐还原作用的缺失,可以将这些菌株与 C. sputorum 生物变种区分开来。本文所提供的数据表明,这些菌株代表了 C. sputorum 中的一个新亚种,因此将其命名为 C. sputorum subsp.
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来源期刊
CiteScore
5.20
自引率
21.40%
发文量
426
审稿时长
1 months
期刊介绍: Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.
期刊最新文献
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